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PCR/qPCR system platform

PCR/qPCR system platform
Case Details
PCR system: Realize inhibitor-resistant PCR amplification and direct PCR amplification of blood/blood card; the multiplex amplification system can realize simultaneous amplification of more than 200 primers;

qPCR system: including SYBR Green and probe method.

SYBR Green qPCR Mix has excellent performance in low-copy, high GC/AT samples, and inhibitor resistance;

The probe-based qPCR product line covers conventional, high-sensitivity, multiplex probes, ARMS, methylation, multi-color probe melting curve, and whole blood direct qPCR, and is compatible with all application requirements on the market.

Related application cases

1. Probe melting curve method qPCR amplification reagent

On conventional fluorescence quantitative PCR instruments on the market, up to 20-plex target detection can be achieved in a single tube.
Example of a 10-plex target single-well amplification assay:

2. Blood direct qPCR amplification reagent

Direct amplification of blood samples or simple lysis followed by amplification reduces operational procedures and shortens detection time

Whole blood direct amplification example (wild type, mutant):

3. Methylation qPCR Amplification Reagents

Amplification of genomic DNA and cell-free DNA after bisulfite treatment

Example of 3-plex target concentration gradient amplification:

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