Nucleic acid extraction platform

Magnetic bead-based nucleic acid extraction generally includes the following steps:

Sample lysis: First, the sample to be extracted is lysed to release nucleic acids from cells or other biological substances. This step can be achieved mechanically, chemically or enzymatically.

Magnetic bead binding: After lysis, surface-modified superparamagnetic nanoparticles are added to the sample. These magnetic beads can selectively bind to target nucleic acid molecules to form "nucleic acid-magnetic bead complexes".

Washing: Through the action of an external magnetic field, the magnetic beads and nucleic acid complexes are aggregated and separated. Then, a washing solution is used to remove impurities in the sample solution, such as proteins, polysaccharides, and salt ions.

Elution: Under low salt and high pH conditions, nucleic acids are dissociated from magnetic beads to obtain a high-purity nucleic acid solution.

Magnetic bead-based nucleic acid extraction has many advantages, such as high efficiency, high purity, easy operation, high degree of automation, and no need to use toxic organic solvents, so it is safer. In addition, this method is suitable for a variety of sample types, including blood, saliva, tissue, environmental samples, etc., and can be used with automated nucleic acid extractors to achieve high-throughput operations.

Magnetic bead-based nucleic acid extraction technology has been widely used in genomics research, medical testing, forensics, and pathogen detection due to its high efficiency, safety, and flexibility.

(II) Nucleic acid extraction by membrane column method

Membrane column nucleic acid extraction is a nucleic acid extraction method that uses the principle of solid-state binding. It adsorbs DNA onto the adsorption membrane (such as glass cellulose membrane) of the centrifugal column and uses centrifugal force to remove impurities such as protein and RNA. This method is easy to operate, suitable for a variety of sample types, and can obtain high-quality DNA, which is suitable for subsequent molecular biology experiments such as PCR, enzyme digestion and hybridization.

Silica gel membrane column is a commonly used adsorption column in membrane column method. The silanol groups on its surface specifically bind to nucleic acids under high salt concentration conditions, and can release the adsorbed nucleic acids under low salt conditions, thereby achieving rapid separation and purification of nucleic acids. This method does not require the use of toxic organic solvents, so it is safer and more environmentally friendly.

Membrane column nucleic acid extraction is widely used in various clinical samples and animal whole blood samples. It can efficiently extract viral RNA and DNA and is suitable for large-scale and high-throughput processing. In addition, this method has also been used to separate nucleic acids less than 300 kilobase pairs from different sources such as cell lysate, plasmid and genomic DNA lysis culture.

Membrane column method for nucleic acid extraction has been widely used in modern molecular biology experiments due to its simplicity, rapidity and high efficiency.

Schematic diagram of the membrane column method for nucleic acid extraction

Qihengxing develops nucleic acid extraction reagents based on magnetic bead method/membrane column method for nucleic acid extraction, and currently covers samples such as whole blood fully automated DNA, plasma cfDNA, FFPE DNA, Escherichia coli plasmid, animal/plant tissue DNA and RNA.

Advantages and Features

1. High purity - the extracted product has good specificity, few inhibitory factors, and is friendly to downstream applications;

2. Automatic operation can be realized-compatible with various automation equipment on the market;

3. Personalized customization - personalized customization services can be provided according to customer needs;

4. Can handle complex samples - plasma, FFPE, soil, feces, rat tail, polysaccharide and polyphenol plants, etc.