| PCR, also known as polymerase chain reaction, is similar to the natural replication process of DNA. Its specificity depends on oligonucleotide primers that are complementary to the two ends of the target sequence. PCR consists of three basic reaction steps: denaturation-annealing-extension. This article details the experimental principles, experimental steps, primer design, result analysis, and the differences, experimental methods, and common problems of various PCRs (RT-PCR, Realtime-PCR, QPCR). |
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PCR, also known as polymerase chain reaction, is similar to the natural replication process of DNA. Its specificity depends on oligonucleotide primers that are complementary to the two ends of the target sequence. PCR consists of three basic reaction steps: denaturation-annealing-extension. This article details the experimental principles, experimental steps, primer design, result analysis, and the differences, experimental methods, and common problems of various PCRs (RT-PCR, Realtime-PCR, QPCR). |
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1. PCR Experiment Basics PCR Experiment Principles and Steps How to design PCR primers online Teach you how to design multiplex PCR primers in 4 minutes A large collection of commonly used primer design tools Preparation of PCR reaction template How to choose PCR reaction conditions Effects of denaturation and annealing temperatures on PCR thermal cycling |
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2. Analysis of PCR Experimental Results |
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From primer design to result analysis, it is very detailed. |
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Teach you how to design primers step by step (with pictures and text) For beginners: Understanding PCR results in one article (highly recommended) Long fragment PCR technology experience sharing What should I do if there is no product in RT-PCR? qRT-PCR precautions compiled by my senior sister with great effort |
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3. Easily teach you how to handle various PCR How many students can distinguish between RT-PCR, Realtime-PCR, and QPCR? How many students are still confused? The following is a detailed summary of various PCRs to help you clear the fog of PCR. |
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RT-PCR, qPCR, Realtime-PCR, can you really tell the difference? RT-PCR Principles and Experimental Techniques qPCR failure, the culprit turned out to be Real-time qPCR Handbook - A step-by-step guide to help you go from beginner to expert |
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Reprinted from Dingxiangtong |
