| PCR, also known as polymerase chain reaction, is based on principles similar to the natural replication process of DNA. Its specificity relies on oligonucleotide primers that are complementary to the target sequence at both ends. PCR consists of three basic reaction steps: denaturation, annealing, and extension. This article details the principles and procedures of PCR experiments, primer design, and result analysis, as well as the differences between various PCR methods (RT-PCR, real-time PCR, and QPCR), experimental methods, and frequently asked questions. |
|
PCR, also known as polymerase chain reaction, is based on principles similar to the natural replication process of DNA. Its specificity relies on oligonucleotide primers that are complementary to the target sequence at both ends. PCR consists of three basic reaction steps: denaturation, annealing, and extension. This article details the principles and procedures of PCR experiments, primer design, and result analysis, as well as the differences between various PCR methods (RT-PCR, real-time PCR, and QPCR), experimental methods, and frequently asked questions. |
|
1. PCR Experiment Basics PCR experimental principles and steps How to design PCR primers online Teach you how to design multiplex PCR primers in 4 minutes A large collection of commonly used primer design tools Preparation of PCR reaction template How to choose PCR reaction conditions Effects of denaturation and annealing temperature on PCR thermal cycling |
|
2. PCR Experimental Results Analysis |
|
From primer design to result analysis, it is very detailed. |
|
A step-by-step guide to primer design (with illustrations and text) Dedicated to beginners: Understanding PCR results in one article (strongly recommended) Sharing long-range PCR technology experience What should I do if there is no product in RT-PCR? qRT-PCR precautions compiled by my senior sister with great effort |
|
3. Easily teach you how to handle various PCR How many students can distinguish between RT-PCR, Realtime-PCR, and QPCR? How many are still confused? Below is a detailed overview of various PCR methods to help you decipher the mysteries of PCR. |
|
RT-PCR, qPCR, Realtime-PCR, can you really tell the difference? RT-PCR Principles and Experimental Techniques Common Problems with qPCR and Its Analysis qPCR failure, the culprit turned out to be Real-time qPCR Handbook—Step-by-step Guide from Novice to Expert |
|
Reprinted from Dingxiangtong |
