StarLighter Blood Direct PCR Mix uses a unique PCR buffer system that can effectively reduce the effects of inhibitors in the blood on amplification, without the need for DNA purification or sample processing. Direct PCR using whole blood samples as template .
This kit is suitable for EDTA anticoagulated blood samples under different storage conditions. Single or multiplex direct blood amplification .
Technology Matrix
No DNA extraction required
StarLight Blood Direct PCR Mix is a 2× ready-to-use PCR premix containing a highly efficient DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR enhancer and stabilizer. When using, you only need to add template, primers and water to perform PCR amplification. .
High inhibitor tolerance
The amount of EDTA anticoagulated blood can be up to 30% of the total reaction volume
Compatible with different amplification primers
Ideal amplification can be achieved for primer types with different GC%/Tm values within 1kb
Wide coverage of sample types
Suitable for amplification of fresh blood, EDTA anticoagulated blood samples stored at 4°C, or frozen at -20°C
Application
1. Direct PCR amplification with EDTA anticoagulated blood;
2. Detection of genomic DNA fragments;
3. High-throughput genetic analysis and genotyping.
Applicable scenarios:
"Emergency blood sampling and testing" in the clinical diagnosis room;
The genetic testing center "processes thousands of samples per day";
Forensic laboratory "Precise analysis of aged blood samples"
Competitive product comparison evidence
Suitable for multiple amplification, compatible with templates with different GC% content, good amplification uniformity, and wide range of blood input volume
Fig.1 Electrophoresis results of hexaplex primer amplification using different proportions of fresh blood added to the blood direct amplification kits from Qihengxing and supplier T.
The blood direct amplification kits from Qihengxing and supplier T were used to perform direct amplification with 6 primers on fresh EDTA anticoagulated blood in different proportions, namely 5%, 10%, 15%, 20%, 25%, and 30%. The amplified fragment ranged from 200 to 800 bp, and the GC% ranged from 35% to 77%. After 35 cycles of amplification, the PCR products were detected by agarose gel electrophoresis.
The results show that Qihengxing Blood Direct Amplification Kit is superior to supplier T in terms of product quantity and amplification uniformity. .
Extreme Challenge Record:
Experimental protocol
Result analysis:
Fig.2 Results of direct amplification of EDTA anticoagulated blood stored at room temperature
Fig.3 Results of direct amplification of EDTA anticoagulated blood at 4℃ storage time
The results showed that for EDTA blood samples stored at different conditions such as room temperature, 4°C, and -20°C, the Qihengxing Blood Direct Amplification Kit can accurately amplify the products of six items.
Product details consultation/product trial application:
010-62149251/15801217100 (same number on WeChat)
