StarLighter Multiplex PCR Mix |
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Multiplex polymerase chain reaction (MPCR), also known as multiplex PCR, is a novel PCR amplification technique developed and improved upon conventional PCR. It allows for the simultaneous amplification of multiple nucleic acid fragments by incorporating two or more primer pairs into a single reaction system. First proposed by Chambehian in 1988, MPCR shares the same reaction principles, reagents, and procedures as conventional PCR. It combines the specificity and sensitivity of single PCR with greater speed and cost-effectiveness, offering significant flexibility in the design of primers and PCR reaction conditions. |
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Schematic diagram of multiplex PCR amplification |
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| Therefore, the amplification results during actual multiplex PCR operations are often unsatisfactory. In the next issue, we will discuss how to optimize multiplex PCR amplification to achieve good amplification results. Stay tuned! |
| StarLighter Multiplex PCR Mix |
| Qihengxing StarLighter MultiplexPCR Mix Designed for multiple PCR The kit is designed for amplification. StarLighter Taq Pro DNA polymerase, and complexes designed for multiplex PCR The optimized buffer system can make different sizes and different GC The amplicons with high content can be amplified uniformly and effectively. At the same time, the hot start achieved by the specific antibody enables the enzyme to DNA It is in an inactive state before melting, which effectively avoids non-specific binding and non-specific amplification of primers before the reaction starts. This system performs annealing and extension in one step, which can effectively reduce PCR Reaction time. This system is compatible with purification DNA , rough extraction DNA , blood cards and other samples. |
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Product advantages: Highly efficient amplification and strong ability to resist inhibitory factors: Pure DNA or crude DNA samples, as well as blood cards and other samples can be amplified. Easy to use: Mix format, only template and primers need to be added, and the system configuration is simple. |
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Experimental Case |
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Using human blood cardboard as a template, 40 pairs of Y chromosome primers were used for multiplex amplification. The amplified products were detected by capillary electrophoresis. The structure is shown below: |
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Using human blood cardboard as a model board, use 28 pairs often Chromosome primers conduct Multiplex amplification , amplified product conduct Capillary electrophoresis detection , the structure is as follows The following figure : |
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