New Product Launch | Based on precise fluorescence quantification—StarLighter dsDNA HS Assay Kit

New Product Launch | Based on precise fluorescence quantification—StarLighter dsDNA HS Assay Kit

StarLighter double-stranded DNA high-sensitivity quantitative detection kit (For use with the Qubit® Fluorometer (all models) or Microplate Reader)

Simple Sensitive precise Stablize

StarLighter dsDNA HS Assay Kit is a simple, sensitive and accurate double-stranded DNA (dsDNA) fluorescence quantitative detection kit. This kit is suitable for Qubit fluorometer or fluorescence microplate reader, and has very good linearity for dsDNA in the range of 0.1-100 ng. It has strong anti-interference ability, and can selectively detect dsDNA even in the presence of ssDNA, RNA and monomeric nucleotides, and tolerates high concentrations of proteins, salts, detergents and other pollutants. Compared with the traditional UV detection (A260) method, DNA can be quantified more accurately. The product characteristics of high specificity and high sensitivity are particularly suitable for quantitative detection of libraries during NGS library construction.

Product Ordering Information

Product Advantages

High sensitivity - can accurately quantify dsDNA samples with a concentration of 100pg/μL-100ng/μL;

Strong specificity - specifically binds to dsDNA and is not affected by RNA;

Good tolerance - Tolerant to higher concentrations of salt, urea, ethanol, detergents, proteins or agarose, and can directly quantify PCR amplification products without purifying DNA from the reaction mixture;

Wide linear range - dsDNA determination, good linearity in the range of 0.1ng/μL -100 ng/μL;

Ease of operation & compatibility - The operation is simple. Prepare the fluorescent detection reagent with buffer to make a working solution, then add the dsDNA sample to be tested, and detect it using an ELISA reader or Qubit® fluorometer. The operation can be carried out at room temperature.

Simple testing process

Excellent and stable product performance

StarLighter dsDNA HS Assay Kit VS Life Qubit® dsDNA HS Assay Kit, StarLighter product performance is equivalent to:

Fig. 1A StarLighter dsDNA HS Assay Kit （ FS-T1001 ) Test Results

Fig. 1B StarLighter dsDNA HS Assay Kit （ FS-T1002 ) Test Results

Fig. 1C Life Qubit® dsDNA HS Assay Kit Test Results

Using Life Qubit standards 1 and 2 as test samples and QB buffer as diluent, standards 1 and 2 were diluted into a series of gradients of 10, 5, 2.5, 1.25, 0.625, 0.3125, 0.156, 0.0781, and 0 ng/µL. Test system: 10µL sample + 190µL working solution. The StarLighter Qubit reagent and Thermo Qubit reagent were used to detect 9 dsDNA samples of different concentrations, and the results are shown in Figure 1: StarLighter dsDNA HS Assay Kit FS-T1001, FS-T1002 and Qubit® dsDNA HS Assay Kit (Life) have the same performance, dsDNA samples have a good linear relationship in the range of 0-100ng, and the sample repeatability is good, and there is no difference in performance between the two.

※ Strong anti-pollution ability StarLighter dsDNA HS Assay Kit Quantitative detection kits are used to quantify samples with high concentrations of pollutants. dsDNA , the results were excellent;

Effects of pollutants on dsDNA quantitative detection kits

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