The StarLighter dsDNA HS Assay Kit is a highly sensitive and specific double-stranded DNA fluorescent quantification kit suitable for use with Qubit fluorometers or fluorescent microplate readers. The kit has good linearity for dsDNA in the range of 0.1-100 ng, strong anti-interference ability, can selectively detect dsDNA, is not affected by ssDNA, RNA, and monomeric nucleotides, and can tolerate higher concentrations of proteins, salts, detergents and other contaminants. Compared with traditio

Dec 4th,2024 1082 Views

New Product Launch | StarLighter dsDNA HS Assay Kit for Precise Fluorescence Quantitation

StarLighter Double-stranded DNA Highly Sensitive Quantitative Detection Kit (For use with the Qubit® Fluorometer (all models) or Microplate Reader)

Simple sensitive precise Stablize

The StarLighter dsDNA HS Assay Kit is a simple, sensitive, and accurate fluorescent quantitative detection kit for double-stranded DNA (dsDNA). Suitable for use with Qubit fluorometers or fluorescent microplate readers, this kit exhibits excellent linearity for dsDNA in the range of 0.1-100 ng. It exhibits strong anti-interference capabilities, allowing selective detection of dsDNA even in the presence of ssDNA, RNA, and monomeric nucleotides, and is tolerant to high concentrations of contaminants such as proteins, salts, and detergents. Compared to traditional UV detection (A260) methods, it allows for more accurate DNA quantification. Its high specificity and sensitivity make it particularly suitable for quantitative detection of libraries during NGS library construction.

Product Ordering Information

Product Advantages

High sensitivity - can accurately quantify dsDNA samples with concentrations ranging from 100 pg/μL to 100 ng/μL;

Strong specificity - specifically binds to dsDNA and is not affected by RNA;

Good tolerance - tolerant to higher concentrations of salt, urea, ethanol, detergents, proteins, or agarose, allowing direct quantitative PCR amplification of products without the need to purify DNA from the reaction mixture;

Wide linear range - dsDNA determination shows good linearity in the range of 0.1 ng/μL to 100 ng/μL;

Ease of Use & Compatibility - Easy to use: Prepare the fluorescent detection reagent with buffer to make a working solution, then add the dsDNA sample to be tested. Detection is performed using a microplate reader or Qubit® fluorometer at room temperature.

Simple detection operation process

Excellent and stable product performance

StarLighter dsDNA HS Assay Kit vs. Life Qubit® dsDNA HS Assay Kit: StarLighter product performance is comparable:

Fig. 1A StarLighter dsDNA HS Assay Kit （ FS-T1001 ) Test results

Fig. 1B StarLighter dsDNA HS Assay Kit （ FS-T1002 ) Test results

Fig. 1C Life Qubit® dsDNA HS Assay Kit Test results

Life Qubit Standards 1 and 2 were used as test samples, and QB buffer was used as the diluent. A gradient series of 10, 5, 2.5, 1.25, 0.625, 0.3125, 0.156, 0.0781, and 0 ng/µL was prepared. The test system consisted of 10 µL sample and 190 µL working buffer. Nine dsDNA samples of varying concentrations were assayed using StarLighter Qubit reagent and Thermo Qubit reagent, respectively. The results are shown in Figure 1. The StarLighter dsDNA HS Assay Kit FS-T1001, FS-T1002, and the Qubit® dsDNA HS Assay Kit (Life) demonstrated comparable performance, exhibiting excellent linearity from 0 to 100 ng of dsDNA and consistent sample reproducibility. There was no significant difference in performance between the two assays.

※ Strong anti-pollution ability StarLighter dsDNA HS Assay Kit Quantitative detection kits are used to quantify the presence of high concentrations of pollutants in samples dsDNA , the results showed excellent performance;

Effects of pollutants on dsDNA quantitative detection kits