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Qihengxing High Sensitive Probe Method qPCR Mix |
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Starlighter HS Probe qPCR Mix (Universal) is a ready-to-use high-sensitivity probe-based qPCR Mix developed based on the ultra-high performance hot-start genetically engineered Taq Pro DNA polymerase. The optimized buffer reaction system can effectively reduce non-specific amplification and greatly increase the amplification efficiency of qPCR, making it suitable for ultra-sensitive qPCR detection. This product has been verified by animal single-cell embryo sex detection and can efficiently amplify DNA as low as fg-level templates. The polymerase in this qPCR Mix is blocked with antibodies, and the enzyme activity can be activated at 90°C for 30 s. The DNA polymerase in this product catalyzes the synthesis of DNA in the 5'→3' direction, has 5'→3' exonuclease activity, and has no 3'→5' proofreading exonuclease activity. When the amplified product has a 3'dA protruding end, it only takes 1-10 s to amplify 1kb. It is compatible with the standard and rapid modes of different qPCR instruments. Because this reagent has ultra-high detection sensitivity and anti-inhibitor amplification, it is more suitable for applications in animal disease and environmental detection. |
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Application Case - African Swine Fever qPCR Detection |
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African swine fever (ASF) is an acute, hemorrhagic, and highly contagious disease caused by infection with the African swine fever virus (ASFV). It is characterized by a short course of disease and an acute infection mortality rate of up to 100%. The World Organization for Animal Health (OIE) has listed it as a notifiable animal disease and included it in the list of animal pathogens to be verified under the Convention on the Prohibition of Biological Weapons. my country has listed it as a Category I animal epidemic. |
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The plasmid standard that meets the primary material standard was prepared with the African swine fever virus gene. The original concentration was 10^5 copies/μL for gradient dilution, and the final concentration was 1.2-240000 copies/reaction (theoretical calculation value). The experimental results showed that in a 10 μL reaction system, a single copy/reaction detection sensitivity could be achieved. The virus plasmid was used as the standard curve, and the amplification efficiency and R2 value were both excellent. |
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Compared with the products from supplier B, Qihengxing showed better detection performance in all tested samples. |
