|
Qihengxing High Sensitive Probe qPCR Mix |
|
Starlighter HS Probe qPCR Mix (Universal) is a ready-to-use, highly sensitive probe-based qPCR mix developed based on the ultra-high-performance, hot-start, genetically engineered Taq Pro DNA polymerase. The optimized buffer system effectively reduces nonspecific amplification and significantly increases qPCR efficiency, making it suitable for ultra-sensitive qPCR assays. This product has been validated for sex determination of single-cell animal embryos and can efficiently amplify DNA templates as low as femtograms. The polymerase in this qPCR mix is blocked with an antibody and activated by heating at 90°C or above for 30 seconds. The DNA polymerase in this mix catalyzes DNA synthesis in the 5'→3' direction, exhibiting 5'→3' exonuclease activity but lacking 3'→5' proofreading exonuclease activity. When the amplified product has 3'dA overhangs, amplification of 1 kb takes only 1-10 seconds. It is compatible with standard and rapid modes on various qPCR instruments. Its ultra-high sensitivity and inhibitor-resistance make it ideal for applications such as animal disease and environmental testing. |
|
|
|
Application Case - African Swine Fever qPCR Detection |
|
African swine fever (ASF) is an acute, hemorrhagic, and highly contagious disease caused by the African swine fever virus (ASFV). It is characterized by a short course of illness and an acute mortality rate of up to 100%. The World Organization for Animal Health (OIE) lists it as a notifiable animal disease and on the list of verifiable animal pathogens under the Biological Weapons Convention. my country classifies it as a Category I animal disease. |
|
|
|
|
|
|
|
A plasmid standard meeting primary material standards was prepared using the African swine fever virus gene. A starting concentration of 10⁵ copies/μL was used for serial dilution, resulting in a final concentration of 1.2–240,000 copies/reaction (theoretical value). Experimental results demonstrated a single copy/reaction detection sensitivity in a 10 μL reaction volume. Using the viral plasmid as a standard curve, amplification efficiency and R² values were excellent. |
|
|
|
|
|
|
|
Compared with the products of supplier B, Qihengxing showed better detection performance in all tested samples. |
