There are many methods for determining nucleic acid concentration, but the commonly used methods in laboratories are UV absorption method 、 Fluorescence spectrophotometry 、 qPCR method And other three methods.

To help you understand the differences between these three methods at a glance, we have compiled a detailed summary of their differences in detection principles and specific applications. Please see the table below for details.

Table 1 Comparison of three nucleic acid concentration determination methods

Among these three methods, fluorescence spectrophotometry is particularly suitable for accurate quantification of rare or difficult-to-process precious samples, and is particularly suitable for NGS library construction and nucleic acid concentration determination in PCR experiments.

Fluorescent dyes used in fluorescence spectrophotometric nucleic acid concentration determination experiments emit fluorescent signals only when bound to specific target molecules (DNA, RNA, or protein). Based on this characteristic, and in conjunction with the use of a fluorometer, the concentration of specific target molecules can be easily and quickly determined, thereby achieving accurate quantification of DNA, RNA, or protein.

Qihengxing Biotechnology has developed cost-effective StarLighter Fluorometer , can support the detection needs of single sample or 8 samples, and the detection targets involve various substances such as DNA, RNA, and protein.

Figure 1 StarLighter Fluorometer

Table 2 StarLighter Fluorometer Product List

01. Product Advantages

Strong specificity: It can specifically detect substances such as dsDNA, ssDNA, RNA and proteins without being interfered by other substances;
Fast detection: The average sample detection time is only 3-5 seconds;
Micro samples: Only 1-20 μL of test sample is required;
Accurate detection: High sensitivity, with good detection results for low-concentration nucleic acid and protein samples;
Reagent kit: Provide matching detection reagents to facilitate users' one-stop shopping.

02. Scope of application

NGS Library construction DNA quantification and RNA quantification in
PCR experiments Determination of nucleic acid concentration in ;
In research laboratories Low concentration Detection requirements for nucleic acids and proteins.

03. Experimental process

Wonderful link: StarLighter nucleic acid quantitative detection reagent

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Figure 3 StarLighter RNA HS Assay Kit

Product Features

One of the earliest high-tech enterprises in China to independently develop nucleic acid quantitative detection reagents;
One-stop supply dsDNA, ssDNA, RNA Quantitative detection reagents for different nucleic acid targets;
Available for Fluorometer and/or microplate reader Products for various application scenarios;
ISO13485/ISO9001 Quality control system of quality system standards;
The batch-to-batch difference is small and the product stability is good.