Name: StarPure Soil DNA Extraction Kit
Brand: Beijing Qihengxing Biotechnology Co., Ltd.
SKU: FS-B1501/FS-B1501-S
Availability: InStock
Rating: 5 (1 reviews)

No.FS-B1501/FS-B1501-S

Qihengxing StarPure Magnetic Bead Soil Nucleic Acid Extraction Kit The nucleic acid of microbial communities (such as bacteria and fungi) in the soil can be extracted and purified, and the protein and nucleic acid in plant debris and animal remains in the soil can be effectively filtered. At the same time, impurities such as pigments and humic acid in the soil can be removed to avoid affecting the application of the extracted nucleic acid in downstream detection. This kit can be applied to various types of soil samples, such as organic nutrient soil, sandy soil, red soil, saline-alkali soil, etc. About 4 μg of nucleic acid can be extracted from 500 mg of soil sample, and the extracted and purified nucleic acid product can be directly used for downstream detection, such as PCR, qPCR, NGS, first-generation sequencing, etc.

Item No./Specification/Price:

FS-B1501/100 rxns/￥2700

FS-B1501-S/30 rxns/￥1000

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Product Description

StarPure Soil DNA Extraction Kit

Product Advantages

Wide range of sample applications - compatible with a variety of different soil samples;
Unique grinding and lysis - effectively filter nucleic acids from plant debris and animal remains in the soil;
High purity - effectively remove impurities and inhibitors such as protein and humic acid in the soil;
Flexible choice of manual and automated - compatible with a variety of mainstream fully automatic nucleic acid extractors. Manual extraction can be used when the number of samples is small, which is convenient and fast.

Application

For extraction of soil RNA

Product composition

Product Performance

The sample is suitable for a wide range of different soil types

DNA was extracted from samples of different soil types from different places using the Qihengxing soil DNA extraction reagent and different imported and domestic soil DNA extraction kits, with three replicates for each sample. For most samples, the amount of DNA extracted by the Qihengxing StarPure soil DNA extraction kit was higher than that of kits from other suppliers.

Fig1. Analysis of the amount and purity of DNA extracted from soil samples of different locations and types using different extraction kits.

Samples 1-8 are Beijing-Garden Soil 1, Beijing-Garden Soil 2, Sanya-Sandy Soil, Sanya-Saline-Alkali Soil, Jiangsu-Field Soil, Shanghai-Orchard Soil, Taiyuan-Garden Soil, and Taiyuan-Field Soil respectively.

The extracted DNA has good integrity

Qihengxing StarPure soil DNA The unique grinding and lysis of the extraction kit can effectively filter out nucleic acids from plant debris and animal remains in the soil; the extracted soil DNA It has high purity and good integrity, and can effectively remove impurities and inhibitors such as protein and humic acid in the soil. DNA Extraction kit DNA Extraction, electrophoresis results show that samples of different soil types, DNA The integrity is relatively good.

Fig2. Electrophoresis of DNA extracted from different soil samples

1. Shanxi - Garden soil 2. Shanxi - Field soil 3. Hainan - Saline-alkali soil 4. Hainan - Sandy soil 5. Shanghai - Orchard soil 6. Jiangsu - Field soil 7. Beijing - Garden soil

High extraction efficiency and better data relevance

Different numbers of rice blast fungus spores were added to sterile soil to make the final concentrations of 5000, 10000, 25000, 50000, 100000, 250000 and 500000 spores/500mg soil. DNA was extracted from these soils using Qihengxing, imported and domestic brand soil extraction kits, eluted with 50μl of elution buffer, and 4μl of the extracted DNA was used for qPCR detection using Qihengxing StarLighter SYBR Green qPCR Mix (FS-Q1002). The Ct value results are shown in Table 1. Qihengxing soil extraction kit showed good extraction efficiency and better input-output correlation (higher R² ).