Product Advantages
Product Application The concentration of the library to be tested is calculated by absolute quantification method and used for library determination before NGS sequencing. Product components  Product Performance 1. The standard product has good quantitative repeatability and a wide concentration range.
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| Tab.1 Comparison of Ct values of different batches of standard products from supplier K and Qihengxing standard products using different instruments Note: Batch 1: 10 plates * 5 replicates/plate; Batch 2: 4 plates * 5 replicates/plate; Batch 3: 2 plates * 10 replicates/plate; Batch 4: 2 plates * 10 replicates/plate. |
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| Tab.2: Both Supplier K and the Qihengxing Library Quantification Kit contain six standards, offering a wide quantification range. Six standards from different batches of Supplier K and the Qihengxing Library Quantification Kit were used as samples and amplified using the same qPCR reagent. The amplification results were then subtracted. Comparison revealed minimal differences between the standards in the two kits, demonstrating comparable performance. The results are shown in the table above. |
| 2. High amplification efficiency and accurate quantitative results |
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| Fig. 2. Competitor standards S4 and S6 were used as samples and amplified using the Qihengxing and competitor kit mixes, respectively. The amplification results were compared with the standard standards in the kit. The quantification results from the Qihengxing and competitor kits were both close to the labeled concentrations. The Qihengxing quantification kit provided accurate and reliable library quantification results. |
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| Tab.3: Standards S4 and S6 from Supplier K were used as samples for amplification and quantification using the Qihengxing and Mix kits from Supplier K, respectively. The quantitative results were compared with the labeled concentrations of the standards in Supplier K's kit. The quantitative results from both the Qihengxing and Supplier K kits were close to the labeled concentrations. The Qihengxing quantification kit provided accurate and reliable library quantification results. |
| Related Product Links NGS-related products (Qubit series/DNA fragment selection & purification/library quantification) |
