Product Advantages
Application The concentration of the library to be tested is calculated by absolute quantification method and used for library determination before NGS sequencing. Product composition  Product Performance 1. The standard product has good quantitative repeatability and a wide concentration range.
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| Tab.1 Comparison of Ct values of different batches of standard products from supplier K and Qihengxing standard products from different instruments Note: Batch 1: 10 plates*5 replicates/plate; Batch 2: 4 plates*5 replicates/plate; Batch 3: 2 plates*10 replicates/plate; Batch 4: 2 plates*10 replicates/plate. |
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| Tab.2 Both Supplier K and Qihengxing Library Quantification Kit contain 6 standards, with a wide quantitative range. Six standards from different batches of Supplier K and Qihengxing Library Quantification Kit were taken as samples, amplified using the same qPCR Mù, and the amplification results were subtracted. The difference between the standards of the two kits was minimal, and the effects were equivalent. The specific results are shown in the table above. |
| 2. High amplification efficiency and accurate quantitative results |
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| Fig.2 Take the standard products S4 and S6 in the competitor's kit as samples, and amplify them with the Mix in the Qihengxing and competitor's kits respectively. The amplified results are compared with the quantitative results of the Qihengxing kit for the standard products in the kit and the quantitative results of the competitor's kit, both of which are close to the labeled concentration. The library quantification results of the Qihengxing quantitative kit are accurate and reliable. |
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| Tab.3 Take the standard products S4 and S6 from supplier K as samples, and use the Mix in the Qihengxing and supplier K kits for amplification quantification, respectively. The quantitative results are compared with the standard products labeled in the supplier K kit. The quantitative results of the Qihengxing kit and the supplier K kit are close to the labeled concentration. The library quantification results of the Qihengxing quantitative kit are accurate and reliable. |
| Related Product Links NGS related products (Qubit series/DNA fragment selection & purification/library quantification) |
