Product Advantages
Product Application Single-strand cDNA synthesis: The synthesized single-strand cDNA can be widely used in downstream experimental applications such as second-strand cDNA synthesis, hybridization, PCR/qPCR, etc. Product components  Product Performance
 Reverse transcription was performed using 40 ng and 1 ng of mouse total RNA as template using StarLighter Color Script RT (FS-P1006) and StarLighter Script RT (FS-P1005), respectively. The resulting cDNA was then quantified by qPCR for ATP and FMR1 using StarLighter HP SYBR Green qPCR Mix (FS-Q1005). FS-P1006 and FS-P1005 showed comparable performance, with no significant differences in amplification linearity, fluorescence intensity, or Ct values.
 StarLighter Color HP SYBR Green qPCR Mix (FS-Q1008) contains a blue dye. cDNA samples reverse-transcribed with StarLighter Color Script RT Super Mix are yellow. When the StarLighter Color HP SYBR Green qPCR Mix is added to the yellow cDNA template, it turns green, enabling tracking of sample addition.
 Reverse transcription was performed using 40 ng and 1 ng of mouse total RNA as template using StarLighter Script RT (FS-P1005) and StarLighter Color Script RT (FS-P1006), respectively. ATP and FMR1 gene expression was quantified by qPCR using StarLighter HP SYBR Green qPCR Mix (FS-Q1005) and StarLighter Color HP SYBR Green qPCR Mix, respectively. The dye-labeled RT plus the dye-labeled qPCR mix (FS-P1006 + FS-Q1008) performed identically to the dye-free RT plus the dye-free qPCR mix (FS-P1005 + FS-Q1005). Amplification linearity, fluorescence intensity, and Ct values were similar. Related Product Links Taq enzyme/DNA enzyme/reverse transcriptase/UDG enzyme |
