StarLighter template tracer high-performance dye-based qPCR master mix

• Convenient pipetting operation - by providing reagents of different colors, the color change effect during pipetting can be used to track the pipetting process and reduce pipetting errors.
• The amplification is highly specific and the data results are reliable;
• High amplification efficiency and early Ct value;
• High signal-to-noise ratio and high sensitivity;
• High GC or high AT fragments have good amplification effects;
• Strong tolerance to inhibitors.

• gene expression analysis;
• Low copy number gene testing;
• Gene knockout validation;
• Gene expression verification;
• RNA copy number detection (virus and bacteria detection)

• Color tracing for easy sample tracking

StarLighter Color HP SYBR Green qPCR Mix contains a blue dye, and 5× Sample Diluent contains a yellow dye. When a sample diluted with Sample Diluent (yellow) is added to StarLighter Color HP SYBR Green qPCR Mix (blue), a color change from blue to green occurs, allowing accurate identification of template addition based on the system color.

• Dyes do not affect the performance of the reagents

Reverse transcription was performed using 40 ng and 1 ng of mouse total RNA as template using StarLighter Color Script RT (FS-P1006) and StarLighter Script RT (FS-P1005), respectively. The resulting cDNA was then quantified by qPCR for ATP and FMR1 using StarLighter HP SYBR Green qPCR Mix (FS-Q1005). FS-P1008 and FS-P1005 showed comparable performance, with no significant differences in amplification linearity, fluorescence intensity, or Ct values.

• Combined with StarLighter Template Tracer High-Performance Dye-Based qPCR Master Mix (FS-Q1008), it enables sample addition and tracing.

• The dyes in RT and SYBR mix do not affect reverse transcription and quantification results.