StarLighter HS Probe qPCR Kit (Universal) This is a probe-based qPCR Kit that uses an ultra-high performance hot-start genetically engineered Taq Pro DNA polymerase configuration. The optimized buffer reaction system can effectively inhibit nonspecific amplification, greatly increase the amplification efficiency of qPCR, and is suitable for ultra-sensitive qPCR detection. This product has been verified by animal embryo sex detection and can efficiently amplify genomic DNA as low as 10 pg of starting template. The polymerase in this qPCR Mix is blocked with antibodies, and the enzyme activity can be restored at above 90°C for 30 seconds. The DNA polymerase in this product catalyzes the synthesis of DNA in the 5'→3' direction, has 5'→3' exonuclease activity, and has no 3'→5' correction exonuclease activity. This product has a 3'-dA protruding end in the amplified product, and the amplification speed is 1-10s/kb. It is compatible with the standard and fast modes of qPCR instruments.
Application
Product composition  Product Performance
Fig.1 Comparison of single cell template amplification results using different reagents The sex of bovine embryos with low starting amount of templates was identified using Qihengxing and competitor products respectively. Qihengxing reagent has higher sensitivity, and the Ct value is 2-4 Ct values earlier than that of competitor KP.
Human genomic DNA was used as a template and diluted continuously to 0.0064 ng-100 ng. There was an excellent linear relationship between the log value of the template amount and the Ct value. 
Fig.2 StarLighter Probe qPCR Mix quantitative results of the template with continuous gradient dilution Using human genomic DNA as template and performing 5-fold gradient dilution (100 ng-0.0064 ng), the Qihengxing reagent can effectively amplify ultra-low concentration templates. Related Product Links |
