Product Advantages
- High sensitivity: dsDNA samples with a concentration of 100pg/μL-100ng/μL can be accurately quantified.
- High specificity: It only binds to dsDNA, has strong specificity, and is not affected by RNA.
- Well tolerated: It tolerates high concentrations of salt, urea, ethanol, detergents, proteins, or agarose, and can directly quantify PCR amplification products without the need to purify DNA from the reaction mixture.
- Ease of operation: The operation is simple. Dilute the fluorescence detection reagent with buffer to make a working solution, then add the dsDNA sample to be tested and detect it using the Qubit fluorescence instrument. This operation can be performed at room temperature.
- More precise quantification: In the presence of ssDNA, RNA, and monomeric nucleotides, dsDNA can be selectively detected at concentrations as low as 100 pg/uL.
Application
dsDNA samples with a concentration of 100pg/μL-100ng/μL can be accurately quantified.
Product composition
Product Performance
Excellent and stable product performance:
StarLighter dsDNA HS Assay Kit VS Competitor L Brand Kit, product performance is comparable:
Fig. 1A StarLighter dsDNA HS Assay Kit test results
Fig. 1B Competitive product L test results
Competitor L standard products 2 and 2 were used as test samples, and QB buffer was used as diluent to dilute standard products 1 and 2 into a series gradient of 10, 5, 2.5, 1.25, 0.625, 0.3125, 0.156, 0.0781, and 0 ng/µL. Test system: 10µL sample + 190µL working solution. For 9 dsDNA samples of different concentrations, the StarLighter Qubit kit competitor L brand kit reagent was used for detection, and the results are shown in Figure 1:
StarLighter dsDNA HS Assay Kit and competitor L have the same performance. The dsDNA samples have a good linear relationship in the range of 0-100ng, and the samples have good repeatability. There is no difference in performance between the two.
The five standards in the enzyme-labeled kit, 10 ng/μL, 5 ng/μL, 2.5 ng/μL, 1.25 ng/μL, and 0 ng/μL, were used as test samples for the calibration curve test. Test system: 10 μL sample + 190 μL working solution. The results are shown in the figure: StarLighter dsDNA HS Assay Kit (Five Standards) has a good linear relationship in the range of 0-100 ng.
Related Product Links
NGS related products (Qubit series/DNA fragment selection & purification/library quantification) |
