• StarLighter 1× dsDNA High Sensitivity Detection Kit
  • StarLighter 1× dsDNA High Sensitivity Detection Kit

StarLighter 1× dsDNA High Sensitivity Detection Kit

No.FS-T1005/FS-T1005-S
Starlighter dsDNA HS Assay Kit This is a simple, rapid, sensitive and highly accurate double-stranded DNA (dsDNA) fluorescence quantitative detection kit. This kit contains fluorescence detection reagents, standards and buffers. It is highly selective for dsDNA, has little RNA interference, and has a good linear relationship between 0.1-100 ng for dDNA samples. Compared with the traditional UV detection (A260) method, it can quantify DNA more accurately, eliminate the interference of other substances, accurately determine the dsDNA concentration, and improve the accuracy of the experiment.
This kit is easy to operate and can be detected using a fluorescent microplate reader or Qubit instrument. In view of the high-throughput detection mode of the microplate reader, FS-T1003 specially provides 5-point standards to make the quantitative results more accurate.
Item No./Specification/Price:
FS-T1005/1250 rxns/¥2000.00
FS-T1005-S/100 rxns/¥200.00
  • StarLighter 1× dsDNA High Sensitivity Detection Kit

Product Description

StarLighter 1× dsDNA HS Assay Kit
StarLighter 1× dsDNA High Sensitivity Detection Kit


Product Advantages

  • High sensitivity: dsDNA samples with a concentration of 100 pg/uL-100 ng/uL can be accurately quantified.
  • Strong specificity: It only binds to dsDNA, has strong specificity, is not affected by RNA, and has excellent tolerance to some common pollutants such as salts, free nucleotides, proteins, solvents, detergents, etc.
  • Good tolerance: It can tolerate higher concentrations of salt, urea, ethanol, detergents, proteins or agarose, and can directly quantify PCR amplification products without purifying DNA from the reaction mixture.
  • Ease of operation: The operation is simple. The premixed 1× working solution can be directly added to the dsDNA sample to be tested and detected by the Qubit fluorometer. This operation can be performed at room temperature.
  • More precise quantification: dsDNA as low as 100pg/uL can be selectively detected in the presence of ssDNA, RNA, and monomeric nucleotides.

Application

dsDNA samples with concentrations ranging from 100 pg/uL to 100 ng/uL can be accurately quantified.

Product composition


Product Performance
  • Excellent and stable performance:

StarLighter dsDNA HS Assay Kit VS Supplier L brand kit, product performance is equivalent to:



Fig.1A Test results of the kit from supplier L

Fig.1B Starlighter dsDNA HS Assay Kit test results
Using supplier L standard products 1 and 2 as test samples, QB buffer as diluent, standard products 1 and 2 were diluted in a series of 10, 5, 2.5, 1.25, 0.625, 0.3125, 0.156, 0.0781, 0 ng/μL. Test system: 10 μL sample + 190 μL working solution. For 9 different concentrations of dsDNA samples, StarLighter Qubit reagent and supplier L brand reagent were used for detection, and the results are shown in Figure 1: StarLighter dsDNA HS Assay Kit and supplier L have the same performance, dsDNA samples have a good linear relationship in the range of 0-100 ng, and the sample repeatability is good, and there is no difference in performance between the two.
The five standards in the enzyme-labeled kit, 10 ng/μL, 5 ng/μL, 2.5 ng/μL, 1.25 ng/μL, and 0 ng/μL, were used as test samples for the standard curve test. Test system: 10 μL sample + 190 μL working solution. The results are shown in the figure: StarLighter dsDNA HS Assay Kit (Five Standards) has a good linear relationship in the range of 0-100 ng.
Related Product Links
NGS related products (Qubit series/DNA fragment selection & purification/library quantification)

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