Product Advantages
Application RNA samples with a concentration of 5-100 ng can be accurately quantified. Product composition |
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Product Performance |
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Fig1 Comparison of the results of continuous gradient dilution template determined by different reagents. |
A. The standard product Standard QS1 with a concentration of 10 ng/μL in the StarLighter RNA HS Assay Kit was gradiently diluted with HS buffer QB to 5 ng/pL, 2.5 ng/L, and 1.25 ng/μL. The fluorescence values of the standards with different concentrations were determined by the StarLighter RNA HS Assay Kit (test system: 10 μL+190 μL working solution). The results are shown in Fig. 1.A. B. The standard product with a concentration of 10 ng/LL in the competitor RNA HS Assay Kit was gradiently diluted with HS buffer Q8 to 5 ng/HL, 2.5 ng/uL, and 1.25 ng/uL. The fluorescence values of the standards with different concentrations were determined by the competitor RNA HS Assay Kit (test system: 10 μL+190 μL working solution). The results are shown in Fig. 1.B. |
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The same RNA samples were measured with StarLighter RNA HS Assay Kit and competitor kit, with two replicates for each sample. The concentration values were averaged and the concentration difference between the two kits was calculated. The results showed that the concentration difference between StarLighter RNA HS Assay Kit and competitor kit was within 5%, and there was no significant difference in performance between the two.
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Table1 StarLighter RNAHSAssay Kit and competitor kits have no difference in RNA sample concentration determination
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