StarLighter dsDNA High Sensitivity Detection Kit V2

• High sensitivity: dsDNA samples with concentrations ranging from 100 pg/uL to 100 ng/pL can be accurately quantified.
• Strong specificity: It only binds to dDNA, has strong specificity, is not affected by RNA, and has excellent tolerance to some common pollutants such as salts, free nucleotides, proteins, solvents, detergents, etc.
• Well tolerated: It tolerates higher concentrations of salt, urea, ethanol, detergents, proteins, or agarose, and can directly quantify PCR amplification products without the need to purify DNA from the reaction mixture.
• Ease of operation: The operation is simple. Dilute the fluorescence detection reagent with buffer to form a working solution, then add the dsDNA sample to be tested and detect it using the Qubit fluorescence instrument. This operation can be performed at room temperature.
• More precise quantification: In the presence of ssDNA, RNA, and monomeric nucleotides, it can selectively detect dsDNA as low as 100 pg/uL.

Excellent and stable product performance:

StarLighter dsDNA HS Assay Kit vs. Competitor L Brand Kit: Product Performance Comparable:

Fig. 1A StarLighter dsDNA HS Assay Kit test results

Fig. 1B Competitive L test results

Competitor L standards 2 and 2 were used as test samples, and QB buffer was used as the diluent. Standards 1 and 2 were diluted to a series of concentrations of 10, 5, 2.5, 1.25, 0.625, 0.3125, 0.156, 0.0781, and 0 ng/µL. The test system consisted of 10µL sample and 190µL working buffer. Nine dsDNA samples of varying concentrations were assayed using the StarLighter Qubit kit from Competitor L. The results are shown in Figure 1.

The StarLighter dsDNA HS Assay Kit and competitor L have identical performance, with good linearity between 0 and 100 ng of dsDNA samples and good sample reproducibility, indicating no performance difference between the two.

The five standards in the ELISA kit (10 ng/μL, 5 ng/μL, 2.5 ng/μL, 1.25 ng/μL, and 0 ng/μL) were used as test samples for a calibration curve. The assay system consisted of 10 μL sample and 190 μL working solution. The results are shown in the figure: The StarLighter dsDNA HS Assay Kit (Five Standards) exhibited excellent linearity in the 0-100 ng range.