Product Recommendation | Ribo-seq Research Artifact-SP-ribo-seq-Pools Ribosomal RNA Removal Kit

Ribo-seq (ribosome sequencing) is a powerful technology that is widely used to study translational regulatory mechanisms and plays an important role in disease research.

By deeply sequencing ribosome-protected mRNA fragments, Ribo-seq is able to capture snapshots of translation activity at specific times and physiological conditions, thereby revealing details of gene expression regulation.

Ribo-seq has been used in the study of cancer, metabolic diseases and other disease areas.

Cancer Research

In cancer research, Ribo-seq is widely used to explore translation regulation, drug resistance, immunotherapy, and nano-drug delivery. It can also be used to reveal abnormally activated translation regulation pathways in cancer cells, thereby providing new targets for cancer treatment. For example, it can reveal the translation regulation of specific mRNAs, thereby enhancing the survival and treatment resistance of melanoma cells; help understand the mechanisms of immunotherapy, such as indoleamine 2 and regulatory pathways.

Metabolic related diseases

In metabolic-related diseases, Ribo-seq found through functional enrichment analysis that differentially expressed genes are mainly enriched in multiple KEGG pathway categories, including metabolism, genetic information processing, cellular processes, tissue systems, and human diseases, etc. This suggests that Ribo-seq may play an important role in the diagnosis and treatment of metabolic-related diseases.

In addition, Ribo-seq has also demonstrated its potential in other disease studies. For example, in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), TDP-43 was identified as a novel mRNA-specific translation enhancer that promotes translation through the 5'UTRs and 3'UTR regions of specific mRNAs.

3. Future applications of Ribo-seq

As a high-resolution and high-throughput technology, Ribo-seq provides a new perspective for understanding RNA regulatory mechanisms and shows great potential in disease research. With the advancement of technology and the improvement of databases, Ribo-seq is expected to become an important tool for the diagnosis and treatment of a variety of diseases.

Table 1 Discovery of Ribo-seq technology in several diseases

Ribosomal RNA (rRNA) accounts for 80-98% of total RNA, which greatly limits the detection efficiency and sensitivity of transcriptomes (such as mRNAs) in RNA-seq. In RNA-seq experiments, it is very necessary to efficiently and specifically remove rRNA to save sample sequencing volume and improve the detection sensitivity of target RNA. .

The Qihengxing SP-ribo-seq-Pools kit is an important tool for Ribo-seq technology, especially performing well in removing ribosomal RNA (rRNA).

The SP-ribo-seq-Pools kit is able to efficiently and specifically bind and remove rRNA by designing a complex biotin-modified DNA probe pool, thereby reducing interference with the target mRNA. Its product advantages are as follows:

1. Applicable to a variety of biological samples, such as humans, mice/rat, fruit flies and nematodes;

2. The operation process is simple and can complete rRNA removal and RNA purification within 60 minutes;

3. Suitable for automation and high-throughput experimental needs;

4. Its capture probe covers the entire rRNA sequence, effectively removing high-abundance and low-abundance rRNA fragments, ensuring the accuracy and repeatability of the experimental results.

In practical applications, the SP-ribo-seq-Pools kit has been proven to significantly improve the detection sensitivity of target mRNA in Ribo-seq experiments, reduce the generation of false positive data, and thus improve the success rate and efficiency of research. Therefore, it can be said that the SP-ribo-seq-Pools kit is a good helper for Ribo-seq technology, providing researchers with a set of scientific, efficient and reliable rRNA removal solutions.

PS: Overview of Qihengxing Ribosomal RNA (rRNA) Removal Kit