Product Recommendation | Ribo-seq Research Artifact - SP-ribo-seq-Pools Ribosomal RNA Removal Kit

Ribo-seq (ribosome sequencing) is a powerful technology that is widely used to study translational regulatory mechanisms and plays an important role in disease research.

By deeply sequencing ribosome-protected mRNA fragments, Ribo-seq can capture a snapshot of translation activity at a specific time and physiological conditions, thereby revealing the details of gene expression regulation.

Ribo-seq has been used in the study of cancer, metabolic diseases and other disease areas.

Cancer Research

In cancer research, Ribo-seq is widely used to explore translational regulation, drug resistance, immunotherapy, and nano-drug delivery. It can also be used to reveal abnormally activated translational regulatory pathways in cancer cells, thereby providing new targets for cancer treatment. For example, it can reveal the translational regulation of specific mRNAs, thereby enhancing the survival and treatment resistance of melanoma cells; and help understand the mechanisms of immunotherapy, such as indoleamine 2 and regulatory pathways.

Metabolic-related diseases

In metabolic-related diseases, Ribo-seq functional enrichment analysis found that differentially expressed genes were mainly enriched in multiple KEGG pathway categories, including metabolism, genetic information processing, cellular processes, tissue systems, and human diseases. This suggests that Ribo-seq may play an important role in the diagnosis and treatment of metabolic-related diseases.

Ribo-seq has also demonstrated its potential in other disease studies. For example, in amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD), TDP-43 was identified as a novel mRNA-specific translation enhancer that promotes translation through the 5'UTRs and 3'UTR regions of specific mRNAs.

3. Future Applications of Ribo-seq

As a high-resolution, high-throughput technology, Ribo-seq provides new insights into RNA regulatory mechanisms and demonstrates tremendous potential in disease research. With advancements in technology and improved databases, Ribo-seq is expected to become an important tool for diagnosing and treating a variety of diseases.

Table 1 Discovery of Ribo-seq technology in several diseases

Ribosomal RNA (rRNA) accounts for 80-98% of total RNA, which greatly limits the detection efficiency and sensitivity of transcriptomes (such as mRNAs) in RNA-seq. In RNA-seq experiments, it is very necessary to remove rRNA efficiently and specifically to save sample sequencing volume and improve the detection sensitivity of target RNA. .

The Qihengxing SP-ribo-seq-Pools kit is an important tool for Ribo-seq technology, especially for removing ribosomal RNA (rRNA).

The SP-ribo-seq-Pools kit, through the design of a complex biotin-modified DNA probe pool, can efficiently and specifically bind to and remove rRNA, thereby reducing interference with the target mRNA. Its product advantages are as follows:

1. Applicable to a variety of biological samples, such as humans, mice/rat, fruit flies, and nematodes;

2. The operation process is simple and can complete rRNA removal and RNA purification within 60 minutes;

3. Suitable for automation and high-throughput experimental needs;

4. Its capture probe covers the entire rRNA sequence, effectively removing high-abundance and low-abundance rRNA fragments, ensuring the accuracy and repeatability of experimental results.

In practical applications, the SP-ribo-seq-Pools kit has been shown to significantly improve the detection sensitivity of target mRNA in Ribo-seq experiments, reduce the generation of false positive data, and thus improve the success rate and efficiency of research. Therefore, it can be said that the SP-ribo-seq-Pools kit is a good helper for Ribo-seq technology, providing researchers with a scientific, efficient, and reliable rRNA removal solution.

PS: Overview of the Qihengxing Ribosomal RNA (rRNA) Removal Kit