The Qihengxing Blood Direct qPCR Kit is designed specifically for blood samples, eliminating the need for complex nucleic acid extraction steps and enabling qPCR amplification with simple lysis. It is suitable for a variety of sample types, including fresh blood, blood stored at 4°C, blood frozen at -20°C, and EDTA-anticoagulated blood. Compatible with Taqman probe-based assays and SNP detection, it offers simple operation and accurate results, completing the entire process from blood sample ana
With the rapid development of molecular diagnostic technology, driven by the demand for rapid, efficient, and low-cost clinical testing and scientific research applications, direct blood amplification (DBA) technology, which does not require nucleic acid extraction, has become a significant development trend. However, existing DBA products often suffer from core issues such as insufficient lysis efficiency leading to reduced amplification sensitivity, a narrow sample application range (inability to cover a variety of samples, including fresh blood, refrigerated blood, and frozen blood), and poor compatibility with primer/probe systems. The key to these issues lies in ensuring reliable and consistent test results while streamlining the operational process.
Leveraging its strengths in nucleic acid amplification technology and reagent development, Qihengxing Bio has developed the StarLighter Blood Direct Amplification Probe qPCR Kit. By optimizing the lysis system and amplification conditions, this kit enables efficient processing of a variety of blood sample types (compatible with complex sample models such as EDTA-anticoagulated blood and frozen blood, while exhibiting excellent compatibility with primers/probes with widely varying GC content and a wide range of Tm values). qPCR testing can be performed directly without the need for complex nucleic acid extraction steps. The test results are comparable to those of traditional nucleic acid extraction methods in terms of fluorescence signal intensity and Ct values, while significantly improving operational convenience and detection speed, providing an innovative solution for blood direct amplification technology that combines both precision and efficiency.
Product Introduction
This kit is applicable to various types of blood samples and does not require tedious nucleic acid extraction and purification. qPCR amplification can be performed by simply lysing the blood sample and taking the supernatant. The operation is simple and can reduce the risk of contamination. The test results are consistent with those obtained by using the pure DNA product after nucleic acid extraction as a template.
Product Advantages
1. Easy operation: heat at 95℃ for 5 minutes and then centrifuge. No complicated blood nucleic acid extraction steps are required.
2. High lysis and release efficiency: the lysis effect of the release agent is consistent with the effect of nucleic acid genome extraction from blood;
3. Wide sample compatibility: Applicable to fresh blood, 4℃ stored blood, -20℃ frozen blood, EDTA anticoagulated blood and other samples;
4. Wide compatibility of primers/probes: Ideal amplification can be achieved for primers/probes with different GC%/Tm values.
Product Application
1. Taqman probe method SNP detection;
2. Quantitative detection by qPCR probe method.
Product Performance
- Simple and fast operation.
It only takes about 1 hour from blood to genotyping/quantification analysis.
Fig.1 Kit operation flow chart
- Accurate SNP typing, compatible with fresh blood, 4 ℃ Storage blood, -20 ℃ Cryopreserved blood Other samples
Genotyping of known SNPs (heterozygous A site: FAM + /Vic - , heterozygous B site: FAM + /Vic - , heterozygous C site: FAM + /Vic - ) was used to perform genotyping on fresh blood sample A, and the test results were accurate (Fig. 2).
Fig.2 Detection results of different SNP sites after lysis and release of fresh blood sample A. The blood was lysed and centrifuged to obtain the supernatant. 4 μL of the supernatant was added to a 20 μL reaction system as a template for qPCR. The test results were consistent with the Sanger sequencing results.
Genotyping of known SNPs (heterozygous A site: FAM + /Vic - , heterozygous B site: FAM + /Vic - , heterozygous C site: FAM + /Vic - ) was genotyped using this kit and the test results were accurate (Fig. 3).
Fig.3 Detection results of different SNP sites after lysis and release of stored blood sample A. The blood was lysed and centrifuged to obtain the supernatant. 4 μL of the supernatant was added to a 20 μL reaction system as a template for qPCR. The test results were consistent with the Sanger sequencing results.
- Fluorescence signal intensity and Ct The value is comparable to that obtained by nucleic acid extraction DNA Amplification results
200 μL of EDTA blood from each sample (A) was treated with the nucleic acid release agent in this kit and the whole blood genomic DNA extraction kit (FS-B8001), respectively. Using the same supernatant/elution volume, 4 μL of each supernatant was used as template for detection of locus B. The results showed that the amplification results after simple whole blood treatment with this kit were consistent with the Ct values obtained after nucleic acid extraction and purification from whole blood, with no change in fluorescence intensity (Fig. 4).
Fig.4 Comparison between Qihengxing blood direct amplification kit and blood nucleic acid extraction kit. The thick line represents the qPCR results after treatment with nucleic acid release solution, and the thin line represents the qPCR results after whole blood genomic DNA extraction and purification. The blue channel represents the FAM channel, the orange channel represents the Rox channel (internal control), and the green channel represents the Vic channel.
Product details/product trial application: 010-62149251/15801217100 (WeChat ID: same)
