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qPCR primer design follows basic PCR principles, but varies depending on the quantification method. For the SYBR dye method, key considerations include primer length, G+C content, 3' end base selection, avoidance of dimers and hairpin structures, Tm matching, ΔG control, 5' end modification, product length, and intron spanning. In addition to these principles, the TaqMan probe method also requires considerations such as avoiding G bases at the 5' end, a higher probe annealing temperature than th

Since its invention in 1985, PCR (polymerase chain reaction) has had a profound impact on life sciences and is widely used in fields such as medical diagnostics and forensic testing. PCR utilizes primers and a thermostable DNA polymerase through three steps: denaturation, annealing, and extension, achieving efficient DNA amplification. A conventional PCR reaction involves a DNA template, primers, DNA polymerase, dNTPs, and a buffer system containing magnesium ions. Modern PCR reagents often come

The StarLighter dsDNA HS Assay Kit is a highly sensitive and specific double-stranded DNA fluorescent quantification kit suitable for use with Qubit fluorometers or fluorescent microplate readers. The kit has good linearity for dsDNA in the range of 0.1-100 ng, strong anti-interference ability, can selectively detect dsDNA, is not affected by ssDNA, RNA, and monomeric nucleotides, and can tolerate higher concentrations of proteins, salts, detergents and other contaminants. Compared with traditio

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Beijing Qihengxing Biotechnology Co., Ltd., established in 2018, is a national high-tech enterprise with multiple invention patents. Leveraging core technology platforms, including enzyme engineering platforms, PCR/qPCR systems, nucleic acid extraction platforms, and NGS platforms, the company has developed numerous high-quality products for diverse applications, including molecular biology, NGS applications, and synthetic biology. The company has also established a platform for technical/practi

How to choose a good probe-based qPCR Mix?