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This article deeply explores the application of new RNA-seq technology in RNA dynamics research. It analyzes the principles, advantages and limitations of Run-on method, PolⅡ immunoprecipitation method, metabolic labeling method and other technical methods, introduces innovative kits to solve traditional problems, provides new ideas for complex transcriptional regulation research, and facilitates the dynamic analysis of gene expression.

In the journey of scientific research, time is the most precious resource, and information is the starlight that illuminates the way forward. In order to help global researchers break through information barriers, Qihengxing Bio has launched a new column for global master, doctoral and postdoctoral recruitment information. Xingzai will regularly collect, organize and update key information such as recruitment and cutting-edge trends of masters, doctors and postdoctoral positions from all over th

Why is the qPCR experiment always interfered by background fluorescence? Negative control shows fluorescence, Ct value jumps? This article deeply analyzes the "quenching code" of 7 types of probes, including TaqMan, molecular beacons, and double hybridization probes, from energy transfer efficiency, probe design misunderstandings to experimental optimization strategies, to help you improve the signal-to-noise ratio and avoid fluorescence traps! Covering scenarios such as SNP detection, fast qPCR

In the journey of scientific research, time is the most precious resource, and information is the starlight that illuminates the way forward. In order to help global researchers break through information barriers, Qihengxing Bio has launched a new column for global master, doctoral and postdoctoral recruitment information. Xingzai will regularly collect, organize and update key information such as recruitment and cutting-edge trends of masters, doctors and postdoctoral positions from all over th

STAR-Click nascent transcript sequencing technology is an innovative method based on click chemistry that can efficiently capture non-coding RNA and transient RNA, solving the capture blind spot and dynamic loss problems of traditional RNA-Seq technology. This technology is widely used in cutting-edge fields such as gene regulation mechanism research, disease biomarker screening, drug screening and mechanism verification, and promotes the development of precision medicine and systems biology.

In the journey of scientific research, time is the most precious resource, and information is the starlight that illuminates the way forward. In order to help global researchers break through information barriers, Qihengxing Bio has launched a new column for global master, doctoral and postdoctoral recruitment information. Xingzai will regularly collect, organize and update key information such as recruitment and cutting-edge trends of masters, doctors and postdoctoral positions from all over th

On March 19, Qihengxing successfully held the first distributor technical training meeting of 2025. 48 national distributors learned in depth about the nucleic acid extraction process, PCR amplification optimization scheme and the core advantages of the kit. Dr. Qin Chubin analyzed the application of molecular diagnostic technology through scenario-based cases to help upgrade terminal services. Qihengxing's annual empowerment plan has been launched, and it will continue to output customized tech

This article deeply explores the two core indicators of molecular diagnostic kits - sensitivity and stability. From the definition, measurement principle to multi-dimensional evaluation method, and then to practical optimization guidelines, it fully reveals how scientists control the quality of the kits, avoid experimental "failures", ensure the reliability of test results, and lay the foundation for precision medicine.