StarPure High Resolution Agarose

StarPure high-resolution agarose has a high resolution for small DNA fragments and is suitable for separating 20bp-50000bp DNA/RNA fragments.

In biochemical experiments, it is often used in gel electrophoresis, two-way immunodiffusion, radial immunodiffusion, preparation of hydrophilic gels, etc.

Product Properties



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Store at room temperature and in a dry place, and transport at room temperature.

1. According to the needs of electrophoresis, prepare electrophoresis and gel-making buffer solutions of appropriate concentrations.

Note: The buffer used for electrophoresis and the buffer used for gel casting must be the same.

2. According to the amount of gel to be prepared and the gel concentration, add accurately weighed agarose powder into a conical flask with a certain amount of electrophoresis buffer (the total liquid volume should not exceed 50% of the capacity of the conical flask).
3. Heat in a microwave to dissolve agarose, heat over medium heat until boiling, keep boiling for about 30 seconds, put on heat-resistant gloves, remove the conical flask and shake carefully to resuspend the undissolved particles, and heat over medium heat again for 1 minute, or until the agarose is completely dissolved. Please wear heat-resistant gloves and shake the conical flask carefully to make the agarose solution fully uniform.

Note: Make sure that the agarose is fully dissolved until the gel solution is clear, otherwise, the electrophoresis image will be blurred. If the gel solution boils and bubbles violently during heating, stop heating. In addition, the heating time in the microwave oven should not be too long.

4. Allow the solution to cool to about 60°C and add ethidium bromide (EB) or GoldView solution according to the instructions. You can also dilute the dye and add it to the sample for better results.

Note: Ethidium bromide is a carcinogen. Wear gloves when working with solutions containing ethidium bromide.

5. Pour the agarose solution into the gel mold and insert the comb in the appropriate position. The thickness of the gel is generally between 3-5mm.
6. When preparing agarose gel with higher concentration, there will be many tiny bubbles in the completely dissolved agarose solution. In this case, after pouring the gel, tap the gel mold on the table a few times to shake out the bubbles. You can then use the tail end of a 200uL gun tip to suck away the bubbles.
7. The gel was allowed to solidify at room temperature (approximately 30 minutes to 1 hour) and then placed in an electrophoresis tank for electrophoresis.

Note: If the gel is not used immediately, please wrap it with plastic wrap and store it at 4°C. It can generally be stored for 2 to 5 days.