StarPure High-Resolution Agarose

StarPure high-resolution agarose has high resolution for small DNA fragments and is suitable for separating 20bp-50000bp DNA/RNA fragments.

In biochemical experiments, it is often used in gel electrophoresis, two-way immunodiffusion, radial immunodiffusion, preparation of hydrophilic gels, etc.

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Store in a dry place at room temperature and transport at room temperature.

1. Prepare electrophoresis and gel-making buffer solutions of appropriate concentrations according to electrophoresis requirements.

Note: The buffer used for electrophoresis and the buffer used for gel casting must be the same.

2. According to the amount of gel to be prepared and the gel concentration, add accurately weighed agarose powder into a conical flask with a certain amount of electrophoresis buffer (the total liquid volume should not exceed 50% of the capacity of the conical flask).
3. Dissolve the agarose in a microwave over medium heat until boiling. Keep the solution boiling for approximately 30 seconds. Wearing heat-resistant gloves, remove the flask and carefully shake it to resuspend any undissolved particles. Heat again over medium heat for 1 minute, or until the agarose is completely dissolved. Wearing heat-resistant gloves, carefully shake the flask to ensure that the agarose solution is thoroughly dissolved.

Note: Ensure that the agarose is fully dissolved until the solution is clear; otherwise, the electrophoresis image will be blurred. If the solution boils and foams violently during heating, stop heating. Also, avoid heating in a microwave for extended periods of time.

4. Cool the solution to about 60°C and add ethidium bromide (EB) or GoldView solution according to the instructions. You can also dilute the dye and add it to the sample for better results.

Note: Ethidium bromide is a carcinogen. Wear gloves when handling solutions containing ethidium bromide.

5. Pour the agarose solution into the gel mold and insert the comb in the appropriate position. The gel thickness is generally between 3-5mm.
6. When preparing agarose gel with a higher concentration, there will be many tiny bubbles in the completely dissolved agarose solution. In this case, after pouring the gel, tap the gel mold on the table a few times to shake out the bubbles. You can then use the tail end of a 200uL pipette to suck away the bubbles.
7. The gel was allowed to solidify at room temperature (approximately 30 minutes to 1 hour) and then placed in an electrophoresis tank for electrophoresis.

Note: If the gel is not used immediately, please wrap it with plastic wrap and store it at 4°C. It can generally be stored for 2 to 5 days.