StarLighter Reverse Transcription Super Master Mix (with gDNA Removal)

• The master mix contains RNA Contains all the components required for first-strand cDNA synthesis except template and water, which is easy to operate and reduces contamination;

• The yield of reverse transcription products is higher;

• Effectively remove gDNA Contamination without affecting reverse transcription performance;

• Wider range of template applications.

• One Chain cDNA synthesis;
• Synthetic single-strand cDNA Can be widely used in 2nd Strand cDNA Synthesis, hybridization, PCR/qPCR And other downstream experimental applications.

Product components


Product Performance

• Effectively remove gDNA, and gDNA contamination does not affect the reverse transcription performance of RNA.

Different starting amounts of mouse,Tota| RNA containing 10% gDNA contamination (0.1, 1, 10, 100, 1000 ng) were processed for gDNA and reverse transcribed, followed by qPCR quantification. The results are shown in the figure.

Fig.1 Evaluation of the Starlighter Reverse Transcription Super Mix (with qDNA Removal) for qDNA removal and its reverse transcription performance on RNA under qDNA contamination

Fig.2 Evaluation of the Starlighter Reverse Transcription Super Mix (with gDNA Removal) for gDNA removal and its reverse transcription performance on RNA under gDNA contamination

• The reverse transcription quantitative results accurately reflect the template amount information, and the RNA input and qPCR quantitative output show an excellent linear relationship.

Fig.3 Different reverse transcription reagents were used to reverse transcribe different starting amounts (0.1, 1, 10, 100, 1000 ng) of mouse Total RNA, and then qPCR was performed to quantify the linear relationship between different reverse transcription reagents and RNA input amount and qPCR Ct value.