StarLighter Reverse Transcription Super Master Mix (with gDNA Removal)

• The master mix contains RNA All components required for first-strand cDNA synthesis except template and water, easy to operate and reduce contamination;

• The yield of reverse transcription products is higher;

• Effective removal of gDNA Contamination without affecting reverse transcription performance;

• Wider range of template applications.

• One Chain cDNA synthesis;
• Synthetic single-strand cDNA Can be widely used in 2nd Strand cDNA Synthesis, hybridization, PCR/qPCR And other downstream experimental applications.

Product composition


Product Performance

• Effectively remove gDNA, and gDNA contamination does not affect the reverse transcription performance of RNA.

Mouse,Tota| RNA with different starting amounts (0.1, 1, 10, 100, 1000 ng) containing 10% gDNA contamination was subjected to gDNA processing and reverse transcription, followed by qPCR quantification. The results are shown in the figure

Fig.1 Evaluation of the qDNA removal ability of Starlighter reverse transcription super premix (including qDNA removal) and the reverse transcription performance of RNA under qDNA contamination1

Fig.2 Evaluation of the gDNA removal ability of Starlighter reverse transcription super premix (including gDNA removal) and the reverse transcription performance of RNA under gDNA contamination

• The reverse transcription quantitative results accurately reflect the template amount information, and the RNA input and qPCR quantitative output show an excellent linear relationship.

Fig.3 Different reverse transcription reagents were used to reverse transcribe mouse Tota| RNA with different starting amounts (0.1, 1, 10, 100, 1000 ng), and then qPCR was performed to quantitatively compare the linear relationship between different reverse transcription reagents on RNA input amount and qPCR Ct value.