Product Advantages
- Strong amplification specificity and reliable data results
- High amplification efficiency, early Ct value
- High signal-to-noise ratio and high sensitivity
- High GC or high AT fragments have good amplification effects
- Strong tolerance to inhibitors
Product Application
- Gene expression analysis
- Low copy number gene detection
- Gene knockout validation
- Gene expression verification
- RNA copy number detection (virus and bacteria detection, etc.)
Product components
Product Performance
- The template has a wide quantitative range, good linear relationship and high amplification sensitivity.
830 pM of the Ion torrent library standard (153 bp) was serially diluted 10-fold and detected using StarLighter High Sensitivity SYBR Green qPCR Mix (Universal). The template amount ranged from 2 × 10 9 When the number of copies is less than 2, the amplification efficiency reaches 99.81%, and the linear relationship R 2 =1.
  
Fig.1 Quantitative range and linear relationship of StarLighter High Sensitivity SYBR Green qPCR Mix (Universal).
- The reagents offer excellent primer and template compatibility and amplification specificity.
StarLighter High Sensitivity SYBR Green qPCR Mix (Universal) can correctly amplify primers with different Tm values, different GC%, and different ΔTm values, as well as amplification products with different Tm values and different GC% values, with standard amplification curves, single melting curves, and no nonspecific amplification.
  
Fig.2 Compatibility and specificity analysis of StarLighter High Sensitivity SYBR Green qPCR Mix (Universal)
- The amplified fluorescent signal is strong and has good specificity.
Different brands of the same type of reagents were used to amplify different concentrations of human gDNA using human CSTB primers. The gDNA template concentrations were 10 ng/pL, 1 ng/pL, 0.1 ng/pL, 0.01 ng/pL, and 0 ng/pL, respectively.
 
 
Fig.3 Comparison of amplification signals of the same type of reagents from different brands
- Strong tolerance to inhibitors.
Different brands of the same type of reagents used human F8-exon-13-F2/R2 primers to amplify gDNA from human FFPE samples with different concentrations. The concentrations of FFPE samples were 10 ng/pL, 1 ng/yL, 0.1 ng/pL, 0.01 ng/pL, and 0 ng/μL, respectively.
 
 
 
Fig.4 Comparison of amplification of gDNA from human FFPE samples with different concentrations using the same type of reagents from different brands
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