Product Advantages
- Strong amplification specificity and reliable data results
- High amplification efficiency, early Ct value
- High signal-to-noise ratio and high sensitivity
- High GC or high AT fragments have good amplification effects
- Strong tolerance to inhibitors
Application
- Gene expression analysis
- Low copy number gene detection
- Gene knockout validation
- Gene expression verification
- RNA copy number detection (virus and bacteria detection, etc.)
Product composition
Product Performance
- The template has a wide quantitative range, good linear relationship and high amplification sensitivity.
The 830 pM Ion torrent library standard (153 bp) was diluted 10-fold and detected using StarLighter High Sensitivity SYBR Green qPCR Mix (Universal) with a template amount range of 2×10 9 When the number of copies is ~2, the amplification efficiency reaches 99.81%, and the linear relationship R 2 =1.
  
Fig.1 Quantitative range and linear relationship of StarLighter High Sensitivity SYBR Green qPCR Mix (Universal).
- The reagents have excellent primer, template compatibility and amplification specificity.
StarLighter High Sensitivity SYBR Green qPCR Mix (Universal) can correctly amplify under different Tm values, different GC%, different ΔTm characteristic primer pairs, different Tm value amplification products, and different GC% amplification products, and the amplification curve is standard, the melting curve is single, and there is no non-specific amplification.
  
Fig.2 Compatibility and specificity analysis of StarLighter High Sensitivity SYBR Green qPCR Mix (Universal)
- The amplified fluorescent signal is strong and has good specificity.
Different brands of the same type of reagents used human CSTB primers to amplify different concentrations of human gDNA. The concentrations of gDNA template were 10 ng/pL, 1 ng/pL, 0.1 ng/pL, 0.01 ng/pL, 0 ng/pL.
 
 
Fig.3 Comparison of amplification signals of the same type of reagents from different brands
- Strong tolerance to inhibitors.
The same type of reagents from different brands used human F8-exon-13-F2/R2 primers to amplify gDNA from human FFPE samples with different concentrations. The concentrations of FFPE samples were 10 ng/pL, 1 ng/yL, 0.1 ng/pL, 0.01 ng/pL, and o ng/μL, respectively.
 
 
 
Fig.4 Comparison of amplification of gDNA from human FFPE samples with different concentrations using the same type of reagents from different brands
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