StarLighter Probe qPCR Master Mix (Universal)

• Strong amplification specificity and reliable data results
• High GC template amplification ability
• High amplification efficiency and early Ct value
• High signal-to-noise ratio and high sensitivity for low copy detection

• Gene expression analysis
• SNP and allele typing
• Validation of sequencing and microarray results

• Strong amplification specificity and reliable data results

Fig.1 Comparison of the accuracy of quantitative results of different reagents

StarLighter Probe qPCR Mix (Universal) and reagents from other suppliers were used to quantify the ApoE gene (ApoE2 and ApoE3) using a human genome containing the ApoE3 genotype (without the ApoE2 genotype) as a template (ARMS-qPCR was used to genotype the two ApoE genotypes, ApoE2 and ApoE3). The StarLighter reagent effectively amplified the ApoE3 genotype (high GC content), while the reagent from supplier K failed to amplify the ApoE gene (amplified fragment length, 197 bp, GC content, 71%).

• Strong amplification ability for high GC templates

Tab.1 Quantification using StarLighter Probe qPCR Mix (Universal) reagent and the human genome carrying ApoE3 gene isoforms as template. Compared with Supplier K, the StarLighter Probe qPCR Mix can detect well, while Supplier K cannot detect.

Fig.2 Comparison of the results of quantification of high GC content fragments using different reagents.

• High amplification efficiency and good linear range

Comparing the quantification of the internal reference gene Actin with StarLighter Probe qPCR Mix (Universal) and Supplier K, the Ct value was earlier than that of Supplier K and the amplification efficiency was better.

Fig.3 Comparison of quantitative results of different reagents for continuous gradient dilution template

• High sensitivity, suitable for low copy detection

Fig.4 StarLighter Probe qPCR Mix quantitative results of low-copy samples

• High stability

Fig.5 StarLighter Probe qPCR Mix stability test results

Reagent A was repeatedly frozen and thawed at least 30 times; reagent B was stored at 37°C for up to 5 days; reagent C was stored at room temperature for up to 28 days; and reagent D was stored at 4°C for up to 43 days. Actin and ApoE genes were quantified using human DNA as a template, and no significant decrease in signal intensity or delay in Ct values was observed (this experiment was performed on a Thermo Q6 instrument).