StarLighter Probe qPCR Master Mix (Universal)

• Strong amplification specificity and reliable data results
• High GC template amplification ability
• High amplification efficiency, early Ct value
• High signal-to-noise ratio and high sensitivity for low copy detection

• Gene expression analysis
• SNP and Allele Typing
• Validation of sequencing and microarray results

• Strong amplification specificity and reliable data results

Fig.1 Comparison of the accuracy of quantitative results of different reagents

StarLighter Probe qPCR Mix (Universal) and other suppliers' reagents were used to quantify the human genome carrying the ApoE3 gene subtype (without the ApoE2 gene subtype) as a template (using ARMS-qPCR technology to type the two ApoE gene subtypes ApoE2 and ApoE3). Qihengxing reagent effectively amplified the ApoE3 gene subtype (high GC), while supplier K failed to amplify the ApoE gene (amplified fragment length 197 bp, GC content 71%).

• Strong amplification ability for high GC templates

Tab.1 Using StarLighter Probe qPCR Mix (Universal) reagent, quantification was performed using the human genome carrying the ApoE3 gene subtype as a template. Compared with supplier K, the StarLighter Probe qPCR Mix can detect well, while supplier K did not detect.

Fig.2 Comparison of the results of different reagents for quantification of high GC content fragments.

• High amplification efficiency and good linear range

The StarLighter Probe qPCR Mix (Universal) was compared with the Supplier K for the quantitative analysis of the internal reference gene Actin. The Ct value was earlier than that of the Supplier K and the amplification efficiency was better.

Fig.3 Comparison of quantitative results of different reagents for continuous gradient dilution template

• High sensitivity, suitable for low copy detection

Fig.4 StarLighter Probe qPCR Mix quantitative low copy number sample results display

• High stability

Fig.5 Results of StarLighter Probe qPCR Mix stability test

Reagent A was repeatedly frozen and thawed at least 30 times; reagent B was stored at 37°C for up to 5 days; reagent C was stored at room temperature for up to 28 days; and reagent D was stored at 4°C for up to 43 days. Using human DNA as a template, Actin and ApoE genes were quantified, and no obvious decrease in signal intensity or delay in Ct value was observed (this experiment was completed on a Thermo Q6 instrument).