StarLighter HS Probe qPCR Kit (Universal) This is a probe-based qPCR Kit that uses an ultra-high performance hot-start genetically engineered Taq Pro DNA polymerization configuration. The optimized buffer reaction system can effectively inhibit nonspecific amplification, greatly increase the amplification efficiency of qPCR, and is suitable for ultra-sensitive qPCR detection. This product has been verified by animal embryo sex detection and can efficiently amplify genomic DNA as low as 10 pg of starting template. The polymerase in this qPCR Mix is blocked with antibodies, and the enzyme activity can be restored at above 90°C for 30 seconds. The DNA polymerase in this product catalyzes the synthesis of DNA in the 5'→3' direction, has 5'→3' exonuclease activity, and has no 3'→5' proofreading exonuclease activity.
This product has 3'-dA overhangs at the end of the amplified product, with an amplification speed of 1-10s/kb. It is compatible with the standard and fast modes of qPCR instruments.
Application
Product composition  Product Performance
Fig.1 Comparison of single cell template amplification results using different reagents The sex of bovine embryos with low starting amount of templates was identified using Qihengxing and competitor products respectively. Qihengxing reagent has higher sensitivity, and the Ct value is 2-4 Ct values earlier than that of competitor KP.
Human genomic DNA was used as a template and diluted continuously to 0.0064 ng-100 ng. There was an excellent linear relationship between the log value of the template amount and the Ct value. 
Fig.2 Quantification results of StarLighter Probe qPCR Mix on the serial gradient dilution template. Using human genomic DNA as template and performing 5-fold gradient dilution (100 ng-0.0064 ng), the Qihengxing reagent can effectively amplify ultra-low concentration templates. |
