StarLighter HS Probe qPCR Kit (Universal) This is a probe-based qPCR Kit that uses an ultra-high-performance hot-start genetically engineered Taq Pro DNA polymerase configuration. The optimized buffer reaction system effectively inhibits nonspecific amplification, greatly increasing the amplification efficiency of qPCR, and is suitable for ultra-sensitive qPCR detection. This product has been validated for animal embryo sex testing and can efficiently amplify genomic DNA with as little as 10 pg of starting template. The polymerase in this qPCR Mix is blocked with antibodies, and enzyme activity can be restored at temperatures above 90°C for 30 seconds. The DNA polymerase in this product catalyzes DNA synthesis in the 5'→3' direction and has 5'→3' exonuclease activity but no 3'→5' proofreading exonuclease activity.
This product has 3'-dA overhangs at the amplified end and an amplification speed of 1-10s/kb. It is compatible with standard and rapid modes of qPCR instruments.
Product Application
Product components  Product Performance
Fig.1 Comparison of single cell template amplification results using different reagents The sex of low-starting-amount template bovine embryos was identified using Qihengxing and a competitor's product. Qihengxing reagent had higher sensitivity, with a Ct value 2-4 Ct values ahead of that of its competitor KP.
Using human genomic DNA as a template, the sample was serially diluted to 0.0064 ng-100 ng. There was an excellent linear relationship between the log value of the template amount and the Ct value. 
Fig.2 Quantification results of StarLighter Probe qPCR Mix on serial gradient dilution template. Using human genomic DNA as a template, the reagent was diluted 5-fold in a series (100 ng-0.0064 ng). The reagent can effectively amplify ultra-low concentration templates. |
