Name: StarLighter probe-based methylation site detection qPCR premix
Brand: Beijing Qihengxing Biotechnology Co., Ltd.
SKU: FS-Q6001-01/FS-Q6001-S
Availability: InStock
Rating: 5 (1 reviews)

No.FS-Q6001-01/FS-Q6001-S

StarLighter Methy-qPCR Mix This qPCR premix is based on a genetically engineered hot-start enzyme and is formulated with an optimized reaction buffer. The high-performance enzyme and optimized reaction buffer system significantly enhance sulfite tolerance, compatibility with diverse sample extraction and conversion products, GC content compatibility (GC content 25%-70%), uracil tolerance, and recognition of 3" mismatches. This significantly improves the sensitivity, specificity, and low-copy detection rate of multiple targets in DNA methylation applications. Results are highly reproducible, and amplification linearity is excellent, enhancing the accuracy of target gene quantification and detection results.

Item No./Specifications/Price:

FS-Q6001-01/5 ml, 4*1.25 ml/box/￥2000

FS-Q6001-S/1 ml, 1 tube/pack/￥500

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Product Description

StarLighter Methy-qPCR Ready Mix
StarLighter probe-based methylation site detection qPCR premix

Product Advantages

Methylation qPCR dedicated premix: The directed modified enzyme and optimized buffer system are suitable for methylation qPCR detection of sulfite-converted DNA templates. It is sulfite-resistant and U-resistant, and has high detection sensitivity.
Wide GC compatibility: It is widely compatible with targets with a GC content range of 20% to 70%.
Good sample adaptability: Suitable for extracting and transforming products from common samples such as fecal extracts, exfoliated cells, blood, serum/plasma, and plasmids, and can be used for the detection of multiple cancer types.
High fluorescence value: stronger fluorescence signal and better amplification curve morphology.

Product Application

Methylation site detection
Low copy gene detection
SNP and allele typing

Product components

Product Performance

Compatible with multi-target detection, strong fluorescence signal and high sensitivity.

The template was a mixed DNA (10% sulfite-converted extracted genomic DNA + 90% unconverted genomic DNA); the GC content of the four target primers ranged from 20% to 60%. ROX fluorescent markers served as internal reference genes (capable of detecting both before and after conversion), and Fam, Vic, and CY5 were used as detection sites.

Wide GC compatibility and good linear amplification curve

Related Product Links

qPCR Product Series

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