Name: StarLighter probe-based methylation site detection qPCR premix
Brand: Beijing Qihengxing Biotechnology Co., Ltd.
SKU: FS-Q6001-01/FS-Q6001-S
Availability: InStock
Rating: 5 (1 reviews)

No.FS-Q6001-01/FS-Q6001-S

StarLighter Methy-qPCR Mix It is a qPCR premix based on genetic engineering hot-start enzyme and optimized reaction buffer. The high-performance enzyme and optimized reaction buffer system greatly improve its tolerance to sulfite, applicability of different sample extraction and conversion products, compatibility of GC content (GC content 25%-70%), resistance to uracil and 3" end mismatch base recognition ability, which significantly improves the sensitivity, specificity and low copy detection rate of multiple targets in DNA methylation application scenarios. The results are reproducible and the amplification line shape is excellent, which improves the accuracy of target gene quantitative results and detection results.

Item No./Specification/Price:

FS-Q6001-01/5 ml, 4*1.25 ml/box/￥2000

FS-Q6001-S/1 ml, 1 tube/pack/￥500

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Product Description

StarLighter Methy-qPCR Ready Mix
StarLighter probe-based methylation site detection qPCR premix

Product Advantages

Methylation qPCR dedicated premix: Directly modified enzymes and optimized buffer system are suitable for sulfite-converted DNA template methylation qPCR detection, sulfite-resistant, U-resistant, and high detection sensitivity.
Wide GC compatibility: It is widely compatible with targets with a GC content ranging from 20% to 70%.
Good sample adaptability: Suitable for extraction and transformation products of common samples such as fecal extracts, exfoliated cells, blood, serum/plasma, plasmids, etc., and used for multi-cancer detection.
High fluorescence value: stronger fluorescence signal and better amplification curve shape.

Application

Methylation site detection
Low copy gene detection
SNP and allele typing

Product composition

Product Performance

Compatible with multi-target detection, strong fluorescence signal and high sensitivity.

The template is mixed DNA (10% of the extracted genomic DNA converted by sulfite + 90% of the unconverted genomic DNA); the GC content of the four target primers ranges from 20% to 60%. ROX fluorescent marker is the internal reference gene (it can detect the internal reference gene before and after conversion), and Fam, Vic, and CY5 are the detection sites.

Wide GC compatibility and good linearity of amplification curve

Related Product Links

qPCR Product Series

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