• StarLighter Hot Start Taq Pro DNA Polymerase
  • StarLighter Hot Start Taq Pro DNA Polymerase

StarLighter Hot Start Taq Pro DNA Polymerase

No.FS-P3001/FS-P3002/FS-P3003
StarLighter HotStart PCR Mix This ready-to-use PCR mix is formulated with an ultra-high-performance hot-start DNA polymerase. Its optimized buffer system enables efficient amplification of complex and challenging samples. Two PCR mixes are available: one with and one without loading dye. The mix with loading dye allows for direct gel electrophoresis after PCR amplification without adding loading buffer, facilitating electrophoresis without compromising amplification efficiency.
The polymerase in this PCR mix is blocked with antibodies; enzymatic activity is restored by heating above 90°C for 30 seconds. This enzyme catalyzes DNA synthesis in the 5'→3' direction, exhibiting 5'→3' exonuclease activity but lacking 3'→5' proofreading exonuclease activity. This product produces 3'-dA overhangs in the amplified product, with an amplification rate of 1-10 s/kb.
Item No./Specifications/Price:
FS-P3001/100 U/¥300.00
FS-P3002/500 U/¥1200.00
FS-P3003/2500 U/¥4800.00
  • StarLighter Hot Start Taq Pro DNA Polymerase

Product Description

StarLighter HotStart Tag Pro DNA Polymerase

StarLighter Hot Start Taq Pro DNA Polymerase



Product Advantages

  • High amplification efficiency;
  • Rapid expansion rate;
  • Good anti-inhibition performance.

Product Application

  • Genotyping;
  • GMO testing;
  • Knockout analysis.

Product components


Product Performance

The amplification efficiency is good and the specificity is high.

Compared with kits from other suppliers on the market, Qihengxing PCR Mix exhibits superior amplification efficiency and specificity for different amplification product lengths and GC contents.

Fig.1A

Fig.1B

Fig. 1. Electrophoresis of PCR products using 20 ng of human gDNA as template and 35 cycles of amplification using PCR mixes from Qihengxing and other suppliers with six primer pairs (amplification products ranging from 400 to 950 bp). Amplification product length and GC% As shown in the figure above, reagents from suppliers VZ, TG, CW, AB, YS, TK1, and TK2 exhibited nonspecific amplification or no amplification.

Fig. 2. Electrophoresis of PCR products using 20 ng of human gDNA as template and 35 cycles of amplification using PCR mixes from Qihengxing and other suppliers with three primer pairs (amplification products 1200-1400 bp). Amplification product length and GC% As shown in the figure above, reagents from suppliers VZ, TG, CW, AB, YS, TK1, and TK2 exhibited nonspecific amplification or no amplification.


Fig. 3. Electrophoresis of PCR products using 20 ng of human gDNA as template and 35 cycles of amplification using PCR mixes from Qihengxing and other suppliers with four primer pairs (amplification products 2000-3000 bp). Amplification product length and GC% As shown in the figure above, reagents from suppliers VZ, TG, CW, AB, YS, TK1, and TK2 exhibited nonspecific amplification or no amplification.

Related Product Links

Taq enzyme/DNA enzyme/reverse transcriptase/UDG enzyme

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