|
Product Advantages
- High amplification efficiency;
- Rapid expansion rate;
- Good anti-inhibition performance.
Application
- Genotyping;
- GMO testing;
- Knockout analysis.
Product composition
Product Performance
The amplification efficiency is good and the specificity is high.
Compared with kits from other suppliers on the market, Qihengxing PCR Mix exhibits superior amplification efficiency and amplification specificity for different amplification product lengths and GC contents.
Fig.1A
Fig.1B
Fig.1 Using 20 ng human gDNA as template, 6 pairs of primers (amplification product 400-950 bp) were amplified for 35 cycles using PCR Mix from Qihengxing and other suppliers, and the electrophoresis results of PCR products are shown in the figure. Amplification product length and GC% As shown in the above figure, the reagents from suppliers VZ, TG, CW, AB, YS, TK1 and TK2 had non-specific amplification or no amplification.
 Fig.2 Using 20 ng human gDNA as template, 3 pairs of primers (amplification product 1200-1400 bp) were amplified for 35 cycles using PCR Mix from Qihengxing and other suppliers, and the electrophoresis results of PCR products are shown in the figure. Amplification product length and GC% As shown in the above figure, the reagents from suppliers VZ, TG, CW, AB, YS, TK1 and TK2 had non-specific amplification or no amplification.
Fig.3 Using 20 ng human gDNA as template, PCR Mix from Qihengxing and other suppliers were used to amplify 4 pairs of primers (amplification product 2000-3000 bp) for 35 cycles. The electrophoresis results of PCR products are shown in the figure. Amplification product length and GC% As shown in the above figure, the reagents from suppliers VZ, TG, CW, AB, YS, TK1 and TK2 had non-specific amplification or no amplification.
Related Product Links
Taq enzyme/DNA enzyme/reverse transcriptase/UDG enzyme
|
