StarLighter DNase 1 DNase I is an endonuclease that cleaves both single-stranded and double-stranded DNA but lacks RNase activity. It hydrolyzes phosphodiester bonds to produce mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-hydroxyl groups. Its activity is strictly dependent on calcium ions and is activated by magnesium or manganese ions. In the presence of magnesium ions, DNase I cleaves double-stranded DNA at random sites on a single strand. In the presence of manganese ions, DNase I cleaves both strands at the same site, resulting in blunt ends or sticky ends with overhangs of 1-2 nucleotides.
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Fig.1 Starlight DNase Ι Comparison of dsDNA digestion ability with different competitors 
Fig.2 Starlight DNase Ι Comparison of ssDNA digestion ability with different competitors Vendor 1: A domestic brand; Vendor 2: An imported brand Qihengxing DNase Ι Compatible with other brands of DNase Ι The same amount of dsDNA sample and ssDNA sample were digested. The electrophoresis after digestion is shown in the figure. Ι The digestion capacity is comparable to that of imported products.
Table 1. StarLight DNase Ι RNase contamination detection analysis. 100 ng RNA and 100 ng RNA + 500 ng human gDNA were used as reaction templates. 0.5 U DNase Ι , digested at 37℃ for 10 min, and inactivated. RT-qPCR was performed using cDNA primer TBP. DNase Ι Treated RNA and untreated DNase Ι The Ct values of the treated RNA and the RT-qPCR single-added RNA positive control were consistent, indicating that DNase Ι RNase-free Ι pollute.
Fig.3 StarLight DNase Ι Inactivation electrophoresis Treat with 10 mM EDTA DNase Ι , incubated at 65℃ for 15 min to inactivate it, and then added pet 28a plasmid and incubated at 37℃ for 30 min. Agarose gel electrophoresis showed no obvious changes. Taq enzyme/DNA enzyme/reverse transcriptase/UDG enzyme |
