StarLighter DNA Enzyme I

• Recombinase;
• No endogenous RNase, derived from non-animal hosts;
• Treatment with 10 mM EDTA at 65°C for 10 min can completely inactivate the protein.

• Preparation of DNA-free RNA samples;
• Preparation of RNA free of DNA contamination before RT-PCR and RT-qPCR;
• DNase I footprinting was used to study DNA-protein interactions.

Product composition

• Strong digestive ability

Fig.1 Starlight DNase Ι Comparison of dsDNA digestion ability with different competitors

Fig.2 Starlight DNase Ι Comparison of ssDNA digestion ability with different competitors

Vendor 1: a domestic brand; Vendor 2: an imported brand

Qihengxing DNase Ι Compatibility with other brands of DNase Ι The same amount of dsDNA sample and ssDNA sample were digested. The electrophoresis after digestion is shown in the figure. Ι The digestion capacity is comparable to that of imported products.

• No RNase contamination

Table 1. StarLight DNase Ι RNase contamination detection analysis.
***Supplier 3: One-step gDNA removal reagent from an imported brand

100 ng RNA and 100 ng RNA + 500 ng human gDNA were used as reaction templates. 0.5 U DNase Ι , digested at 37℃ for 10 min, and inactivated. RT-qPCR was performed using cDNA primer TBP. DNase Ι Treated RNA and untreated DNase Ι The Ct values of the treated RNA and the RT-qPCR single RNA positive control were consistent, indicating that DNase Ι RNase-free Ι pollute.

• Simple inactivation process

Fig.3 StarLight DNase Ι Inactivation electrophoresis

Treat with 10 mM EDTA DNase Ι , incubated at 65℃ for 15 min to inactivate it, and then added pet 28a plasmid and incubated at 37℃ for 30 min. Agarose gel electrophoresis showed no obvious changes.