StarLighter Hot Start Taq Pro DNA Polymerase (Glycerol-free)

Product Introduction

StarLighter Hot Start Taq Pro DNA Polymerase has been optimized for rapid and efficient synthesis of large amounts of DNA while remaining resistant to various inhibitors. It can be used directly for rapid amplification, multiplex amplification, and other amplifications of crude samples. This enzyme allows for room temperature reaction setup. Because the enzyme is blocked with an antibody and requires exposure to temperatures above 90°C for 30 seconds to restore enzymatic activity, it is inactive at room temperature, effectively preventing primer-dimer formation and nonspecific extension, thereby increasing the specificity of DNA amplification. The enzyme exhibits 5'→3' exonuclease activity but lacks 3'→5' proofreading exonuclease activity.

This product has 3'-dA overhangs in the amplified product and can be directly cloned into a TA vector after purification. The amplification speed is 1 ~ 10 s/kb.

This product is glycerol-free and can be used in the development of freeze-dried reagents.

Product components

Storage and transportation conditions

Store at -25 ~ -15℃, transport at < 0℃.

Product Application

Direct expansion of bacterial liquid;

Amplification of crude samples;

Other PCR rapid amplification, etc.

Features

Remarkably resistant to various inhibitory factors;

Effectively amplify templates with high GC content and complex secondary structures;

Time-saving and efficient: It can be used for direct amplification of bacterial suspensions, colonies, and crude extracts.

Experimental Procedure

1. System preparation (taking 20 μL reaction system as an example)
   • Before using the reagents, ensure that they are thoroughly melted and mixed. All templates, primers and other components added to the reaction system should also be fully mixed.
   • Calculate the volume of each component to be added (refer to the table below) and carefully pipette the exact volume into the PCR reaction tube;

1. The final concentration of primers in the reaction can be adjusted between 1 and 1 μM.
2. If the template is genomic DNA, the recommended addition amount is 10 to 100 ng. If the template is plasmid or PCR product DNA, the recommended addition amount is < 10 ng.
   • Close the tube cap and centrifuge briefly.
3. Recommended PCR reaction program:

1. The pre-denaturation time is set at 30 sec ~ 5 min. If the template GC content is high, the pre-denaturation time can be appropriately extended to 5 min.
2. Adjust the annealing temperature according to the primer Tm (recommended temperature 55 ~ 65℃).
3. The extension time is determined according to the length of the PCR product.
4. The number of annealing and extension cycles can be adjusted based on the initial template amount and the desired product quantity. Using 4 ng of human genomic DNA (or a 5 mm blood card) as a template, 45 sec extension, and 30 cycles of amplification can achieve approximately 1 μg of product accumulation.