StarRed is a highly sensitive, highly stable and safe fluorescent nucleic acid gel staining reagent. Its unique oily macromolecular structure prevents it from penetrating the cell membrane and entering the cell, and it is not easy to volatilize and sublimate and be inhaled by the human body. StarRed has the same spectral characteristics as ethidium bromide (EtBr, EB). Its unique molecular structure can electrostatically bind to DNA and will not affect the migration of DNA bands. Therefore, it can replace unsafe nucleic acid dyes such as EB and does not require decolorization. It is suitable for dsDNA, ssDNA and RNA staining in agarose and polyacrylamide gel electrophoresis. You can choose gel staining or bubble staining for staining. It is very convenient and flexible to use.
1. If the macromolecular bands are tailing and the separation is not ideal, it is recommended to reduce the amount of DNA marker or nucleic acid sample loaded.
2. The gel staining method is not suitable for prefabricated polyacrylamide gels. For polyacrylamide gels, please use the bubble staining method.
3. For your safety and health, please wear a lab coat and disposable gloves when operating.
4. This product is for scientific research purposes only!
TAE buffer configuration: 50X TAE electrophoresis buffer [Tris 242g (2M), EDTA 37.2g (100mM), add about 57ml of acetic acid to adjust pH=8.5; make up to 1000mL; use ddH 2 Prepare 1X TAE electrophoresis buffer by diluting 50-fold.
TBE buffer configuration: 10X TBE electrophoresis buffer [Tris 107.8146 g (890 mM), boric acid 55.0287 g (890 mM), EDTA 5.845 g (20 mM), add about 4 g of NaOH to adjust pH = 8.3; constant volume 1000 mL];
Prepare 1X TBE electrophoresis buffer by diluting 10-fold with ddH2O.
1) Prepare agarose gel of appropriate concentration and heat it in a microwave oven until it is completely melted.
2) Add GelRed nucleic acid dye to a final concentration of 1× (i.e., add 5 μL of StarRed 10,000× aqueous solution to every 50 mL of agarose solution).
3) Pour the agarose solution containing Star Red nucleic acid dye into the gel casting apparatus and insert the comb. Let it solidify at room temperature for about 30-60 minutes.
4) Load the sample and perform electrophoresis according to conventional methods.
5) Ultraviolet photography and observation.
✮ Note : Star Red has good thermal stability and can be added directly to hot agarose solution. Alternatively, Star Red stock solution can be added to electrophoresis buffer containing agarose powder and then heated in a microwave or other common methods to prepare agarose gel.
Star Red is compatible with all commonly used electrophoresis buffers.
1) Prepare agarose gel of appropriate concentration and heat it in a microwave oven until it is completely melted.
2) Pour the agarose solution into the gel casting apparatus and insert the comb. Let it solidify at room temperature for about 30-60 minutes.
3) Load the sample and perform electrophoresis according to conventional methods.
4) Dilute the StarRed 10,000× aqueous solution with 0.1 M NaCl solution to a 3× staining solution (i.e., add 15 μL of StarRed 10,000× aqueous solution to 50 mL of 0.1 M NaCl solution. The staining solution can be reused about 3 times and should be stored at room temperature away from light).
5) Place the gel in a suitable container and add 3× staining solution to submerge the gel. Stain at room temperature for about 30 minutes with shaking. The optimal staining time is related to the thickness and concentration of the gel. For gels containing 3.5-10% polyacrylamide, the staining time is usually between 30 minutes and 1 hour, and increases with the increase of polyacrylamide content.
