StarRed is a highly sensitive, stable, and safe fluorescent nucleic acid gel stain. Its unique oily macromolecular structure prevents it from penetrating cell membranes and entering cells, and it also resists volatilization and sublimation, making it less susceptible to absorption. StarRed shares spectral characteristics with ethidium bromide (EtBr, EB). Its unique molecular structure allows for electrostatic binding to DNA without affecting DNA band migration, making it a viable alternative to less-safe nucleic acid dyes like EB and eliminating the need for destaining. StarRed is suitable for staining dsDNA, ssDNA, and RNA in agarose and polyacrylamide gel electrophoresis, with either gel staining or bubble staining methods available, offering exceptional convenience and flexibility.
1. If large molecular bands are tailing and separation is unsatisfactory, it is recommended to reduce the amount of DNA marker or nucleic acid sample loaded.
2. The gel staining method is not suitable for precast polyacrylamide gels. For polyacrylamide gels, please use the bubble staining method.
3. For your safety and health, please wear a lab coat and disposable gloves when operating.
4. This product is for scientific research purposes only!
TAE buffer preparation: 50X TAE electrophoresis buffer [Tris 242g (2M), EDTA 37.2g (100mM), add about 57ml of acetic acid to adjust the pH to 8.5; make up to 1000mL; use ddH 2 Prepare 1X TAE running buffer by diluting the solution 50-fold.
TBE buffer preparation: 10X TBE electrophoresis buffer [Tris 107.8146 g (890 mM), boric acid 55.0287 g (890 mM), EDTA 5.845 g (20 mM), add approximately 4 g of NaOH to adjust pH to 8.3; make up to 1000 mL];
Prepare 1X TBE running buffer by diluting the sample 10-fold with ddH2O.
1) Prepare agarose gel of appropriate concentration and heat in a microwave oven until completely melted.
2) Add GelRed nucleic acid dye to a final concentration of 1× (i.e., add 5 μL of StarRed 10,000× aqueous solution to every 50 mL of agarose solution).
3) Pour the agarose solution containing Star Red nucleic acid dye into the gel casting apparatus and insert the comb. Let it solidify at room temperature for about 30-60 minutes.
4) Load the sample and perform electrophoresis according to conventional methods.
5) Take ultraviolet photos and observe.
✮ Note : Star Red has good thermal stability and can be added directly to hot agarose solution. Alternatively, Star Red stock solution can be added to electrophoresis buffer containing agarose powder and then heated in a microwave or other common methods to prepare agarose gel.
Star Red is compatible with all commonly used electrophoresis buffers.
1) Prepare agarose gel of appropriate concentration and heat in a microwave oven until completely melted.
2) Pour the agarose solution into the gel casting apparatus and insert the comb. Let it solidify at room temperature for about 30-60 minutes.
3) Load the sample and perform electrophoresis according to conventional methods.
4) Dilute the StarRed 10,000× aqueous solution with 0.1 M NaCl solution to a 3× staining solution (i.e., add 15 μL of StarRed 10,000× aqueous solution to 50 mL of 0.1 M NaCl solution. This staining solution can be reused about three times and should be stored at room temperature in the dark).
5) Place the gel in a suitable container and add 3x staining solution to submerge the gel. Stain at room temperature with shaking for approximately 30 minutes. The optimal staining time depends on gel thickness and concentration. For gels containing 3.5-10% polyacrylamide, staining time typically ranges from 30 minutes to 1 hour, and increases with increasing polyacrylamide content.
