New Product | StarLighter PDX Model Mouse Cell Infiltration Status Detection Kit

Patient-Derived tumor Xenograft (PDX) is a humanized xenograft model established by directly transplanting the patient's tumor tissue into immunodeficient mice. The PDX model can retain the genetic diversity and microenvironment of the primary tumor, better simulate the actual situation of the tumor in the patient's body, and is a useful tool for cancer mechanism research and drug testing.

However, as the number of passages increases, the fidelity of tumor tissue decreases. To ensure that the model remains consistent during the passage process and beyond, rigorous validation and quality control are important.

The StarLighter PDX Model Mouse Cell Infiltration Status Detection Kit uses a dual-color fluorescent probe and a double standard curve absolute quantitative qPCR method to detect the proportion of mouse DNA in the total DNA of the PDX model, and deduce the mouse cell infiltration ratio based on this. It is an effective tool for PDX model quality control.

• A quantitative test kit for human and mouse nucleic acid detection with independent intellectual property rights;

• Double standard curve absolute quantitative qPCR method, the results are accurate and reliable;

• Wide quantitative range, suitable for nucleic acid concentration > 1ng/ μ L mixed nucleic acids of different ratios of human and mouse;

• The minimum value of the standard curve is 10 ³ copy/μL, and can accurately quantify mixed nucleic acids with a human nucleic acid ratio as low as 0.5%.

02. Product compatibility

• The amplification efficiency of PDX model nucleic acids extracted by different nucleic acid extraction methods may be different, but it has no effect on the ratio of human and mouse nucleic acids.

• When using qPCR instruments of different brands, it is necessary to select the appropriate Rox according to the instrument's requirements for the reference dye Rox.

1. It will contain 10 ⁸ , 10 ⁷ , 10 ⁶ , 10 ⁵ , 10 ⁴ , 10 ³ Two plasmid DNA copies of mouse and human gene targets were used as standards for fluorescence quantification and a standard curve was drawn.

Fig.1 10 ³ -10 ⁸ Standard curve and amplification curve of mouse and human plasmids

Second, the mixed simulated samples of mouse and human plasmid DNA in different ratios were tested (mixed ratios were: 1:99, 10:90, 25:75, 50:50), and 10 ⁵ , 10 ⁴ , 10 ³ The copy number was used as the sample input for qPCR quantification, and the ratio of mouse and human genes in the mixed sample was calculated based on the standard curve.

Fig.2 Test results of mixed simulated samples of different mouse and human plasmid DNA

3. Test simulated samples of mouse and human genomic DNA mixed in different ratios (mixed ratios are: 1:99, 10:90, 25:75, 50:50), use 10ng, 25ng and 100ng as sample input for qPCR quantification, and calculate the ratio of mouse and human genes in the mixed samples according to the standard curve.

in conclusion:

The StarLighter PDX model mouse cell infiltration status detection kit has accurate quantitative results and can accurately calculate the mouse and human DNA ratios for different ratios of mouse and human plasmid DNA samples and different ratios of mouse and human genomic DNA samples.