New Product | StarLighter PDX Model Mouse Cell Infiltration Status Detection Kit

Patient-Derived Tumor Xenograft (PDX) models are established by directly transplanting patient tumor tissue into immunodeficient mice. PDX models can retain the genetic diversity and microenvironment of the primary tumor, better simulating the actual situation of the tumor in the patient's body, and are useful tools for cancer mechanism research and drug testing.

However, tumor tissue fidelity decreases with increasing passage number. To ensure model consistency during passage and beyond, rigorous validation and quality control are important.

The StarLighter PDX Model Mouse Cell Infiltration Status Detection Kit uses a dual-color fluorescent probe and a dual-standard curve absolute quantitative qPCR method to detect the ratio of mouse DNA in the total DNA of the PDX model, and derive the mouse cell infiltration ratio from this, making it an effective tool for PDX model quality control.

• Quantitative detection kits for human and mouse nucleic acids with independent intellectual property rights;

• Double standard curve absolute quantitative qPCR method, the results are accurate and reliable;

• Wide quantitative range, suitable for nucleic acid concentration >1ng/ μ L mixed nucleic acids of human and mouse in different ratios;

• The lowest value of the standard curve is 10 ³ copy/μL, it can accurately quantify mixed nucleic acids with a human nucleic acid ratio as low as 0.5%.

02. Product compatibility

• The amplification efficiency of PDX model nucleic acids extracted using different nucleic acid extraction methods may vary, but this has no effect on the ratio of human to mouse nucleic acids.

• When using qPCR instruments of different brands, it is necessary to select the appropriate Rox according to the instrument's requirements for the reference dye Rox.

1. Will contain 10 ⁸ , 10 ⁷ , 10 ⁶ , 10 ⁵ , 10 ⁴ , 10 ³ Two plasmid DNA copies of mouse and human gene targets were used as standards for fluorescence quantification and a standard curve was drawn.

Fig.1 With 10 ³ -10 ⁸ Standard curve and amplification curve of mouse and human plasmids

Second, the mixed simulated samples of mouse and human plasmid DNA in different ratios were tested (mixed ratios were: 1:99, 10:90, 25:75, 50:50), and 10 ⁵ , 10 ⁴ , 10 ³ The copy number was used as the sample input for qPCR quantification, and the ratio of mouse and human genes in the mixed sample was calculated based on the standard curve.

Fig.2 Test results of mixed simulated samples of different mouse and human plasmid DNA

3. We tested simulated mixed samples of mouse and human genomic DNA at different ratios (mixing ratios were: 1:99, 10:90, 25:75, and 50:50). qPCR quantification was performed using 10 ng, 25 ng, and 100 ng of sample input, respectively. The ratio of mouse to human genes in the mixed samples was calculated based on the standard curve.

in conclusion:

The StarLighter PDX model mouse cell infiltration status detection kit provides accurate quantitative results and can accurately calculate the mouse:human DNA ratio for samples containing different ratios of mouse:human plasmid DNA and samples containing different ratios of mouse:human genomic DNA.