New Product | StarPure Magnetic Bead Plasmid Extraction Kit

StarPure Magnetic Beads Plasmid Extraction Kit It is a plasmid extraction technology based on the magnetic bead method, combined with an optimized buffer system to achieve the extraction and purification of high-purity plasmid DNA.

The plasmid DNA extracted by this kit can be used in various downstream molecular biology experiments, such as sequencing, library screening, enzyme digestion, ligation and transformation.

This kit does not require the use of toxic organic solvents (such as phenol, chloroform, etc.), the extraction process is simple, and it is suitable for manual and high-throughput automated extraction.

Product Advantages

• High plasmid extraction efficiency and good quality ( High proportion of supercoiled plasmids );

• High purity, no RNA residue;

• Based on magnetic bead method, compatible with automated workstation extraction.

Product Performance

The DH5α strain containing the pGSI plasmid was cultured in LB medium until the OD ₆₀₀ ≈3.80. Extraction was performed using plasmid extraction kits from different manufacturers, with each sample containing 1 mL of bacterial culture and a final plasmid elution volume of 50 μL. The plasmid concentration and yield extracted by Qihengxing were higher than those from other manufacturers.

pGSI plasmid was extracted using kits from different manufacturers. The electrophoresis results showed that the main bands of the Qihengxing extraction product were supercoiled structures, with no genomic and RNA contamination, and the proportion of supercoiled plasmids was high.

Fig.1 Electrophoresis of plasmids from the same sample extracted using plasmid extraction kits from different manufacturers

Fig.2 pGSI LB refers to a DH5α strain containing the pGSI plasmid cultured in LB medium; pGSI SOB refers to a DH5α strain containing the pGSI plasmid cultured in SOB medium; pet28a LB refers to a BL21 strain containing the pet28a plasmid (with cloned expression gene, approximately 6.5 kb in length) cultured in LB medium; high-purity plasmids were extracted from all strains.