| Product Introduction |
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StarPure Magnetic Bead Plasmid Extraction Kit It is a plasmid extraction technology based on the magnetic bead method, combined with an optimized buffer system, to achieve the extraction and purification of high-purity plasmid DNA. The plasmid DNA extracted by this kit can be used in various downstream molecular biology experiments, such as sequencing, library screening, enzyme digestion, ligation and transformation. This kit does not require the use of toxic organic solvents (such as phenol, chloroform, etc.), the extraction process is simple to operate, and is suitable for manual and high-throughput automated extraction. |
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Product Advantages |
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Product Performance |
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01. High yield The DH5α strain containing the pGSI plasmid was cultured in LB medium until OD 600 ≈3.80, using plasmid extraction kits from different manufacturers for extraction, each sample was 1 mL of bacterial solution, and the final plasmid elution volume was 50 μL. The concentration and yield of plasmid extracted by Qihengxing were higher than those of other manufacturers. |
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02. The extracted product is of high purity and free of genomic and RNA contamination pGSI plasmid was extracted using kits from different manufacturers. The electrophoresis results showed that the main bands of the Qihengxing extraction product were supercoiled structures, with no genomic and RNA contamination, and the proportion of supercoiled plasmids was high. |
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Fig.1 Electrophoresis of plasmids from the same sample extracted by plasmid extraction kits from different manufacturers |
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03. Suitable for extraction of different plasmids and different cultures |
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Fig.2 pGSI LB is a DH5α strain containing pGSI plasmid cultured in LB medium; pGSI SOB is a DH5α strain containing pGSI plasmid cultured in SOB medium; pet28a LB is a BL21 strain containing pet28a plasmid (with expression gene cloned, total length about 6.5kb) cultured in LB medium; high-purity plasmids can be extracted from all of them. |
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