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The design of a fluorescent quantitative PCR experiment requires the selection of relative or absolute quantification methods based on the purpose. Relative quantification is used to detect the relative expression differences of genes in different samples. The ΔΔCT method or the relative standard curve method is commonly used. Appropriate internal reference genes must be selected to eliminate human errors. Absolute quantification is used to detect the exact content of genes or species in a sampl

qPCR, due to its simplicity, rapid detection, high sensitivity, and excellent specificity, has become the gold standard for nucleic acid quantification and is widely used in fields such as life sciences, agronomy, molecular diagnostics, and medicine. Nucleic acid extraction and quality control are critical initial steps in qPCR experiments. Proper sample collection and processing, as well as effective nucleic acid extraction and rigorous quality control, are crucial for ensuring accurate experim

Next-generation sequencing (NGS), or massively parallel sequencing, can sequence large numbers of DNA molecules simultaneously. It represents a revolutionary advancement following Sanger sequencing. It boasts high throughput, high sensitivity, and specificity, enabling both qualitative and quantitative detection at a relatively low cost. It holds broad application prospects in areas such as non-invasive prenatal screening and tumor gene mutations, and is a crucial enabling technology in the era

In RT-PCR, efficient conversion of RNA to cDNA is a critical and error-prone step. Before reverse transcription, RNA concentration, purity, and integrity must be considered, as these factors impact reverse transcription efficiency and quantitative accuracy. The input RNA amount should be adjusted according to the experimental situation, adhering to the principle of equal input amount. For low-abundance genes, the input amount can be increased. Genomic DNA contamination can be addressed by DNase

qPCR data analysis includes relative quantification and absolute quantification, with relative quantification being commonly used in laboratories. Absolute quantification uses a standard sample with a known starting copy number to generate a standard curve. The linear relationship between log(starting concentration) and cycle number is used to calculate the amount of template in the sample. This requires determining the target gene, standard sample, replicate reaction wells, and labeling method.

Qihengxing Biotechnology Co., Ltd. is a high-tech enterprise specializing in core raw materials upstream of the molecular biology industry chain. It primarily engages in the research, development, production, and sales of molecular and protease-related reagents. Its products cover a wide range of categories, including qPCR, NGS, PCR, molecular, in vitro transcription, antibodies, protein purification, protein analysis, recombinant proteins, high-throughput sequencing, reporter gene detection, an

Qihengxing Biotechnology Co., Ltd. is a high-tech enterprise specializing in core raw materials upstream of the molecular biology industry chain. It primarily engages in the research, development, production, and sales of molecular and protease-related reagents. Its products cover a wide range of categories, including qPCR, NGS, PCR, molecular, in vitro transcription, antibodies, protein purification, protein analysis, recombinant proteins, high-throughput sequencing, reporter gene detection, an

Multiplex PCR (MPCR) is an extension of conventional PCR. It allows the simultaneous amplification of multiple nucleic acid fragments by adding multiple primer pairs to the same reaction system. It is specific, sensitive, rapid, and economical, and is widely used in various fields. In practice, it is necessary to strike a balance between the amplification conditions of multiple targets to achieve perfect compatibility between primers. The Qihengxing StarLighter Multiplex PCR Mix is specifically