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Qihengxing Biotechnology Co., Ltd. is a high-tech enterprise focusing on the core raw materials in the upstream of the molecular biology industry chain, mainly engaged in the research and development, production and sales of molecular and protease-related reagents. Its R&D products cover multiple categories such as qPCR, NGS, PCR, molecules, in vitro transcription, antibodies, protein purification, protein analysis, recombinant proteins, high-throughput sequencing, reporter gene detection, and k

qPCR data analysis includes relative quantification and absolute quantification, and relative quantification is commonly used in laboratories. Absolute quantification uses a standard sample with a known starting copy number to make a standard curve, and uses the linear relationship between Log (starting concentration) and the number of cycles to calculate the amount of template in the sample. It is necessary to determine the target gene, standard sample, repeated reaction wells, labeling method,

In RT-PCR, the efficient conversion of RNA into cDNA is a critical and error-prone step. Before reverse transcription, the concentration, purity, and integrity of RNA must be considered, as these factors affect the efficiency of reverse transcription and the accuracy of quantification. The starting amount of RNA should be adjusted according to the experimental situation, following the principle of the same starting amount. For low-abundance genes, the starting amount can be increased. Genomic DN

Next-generation sequencing (NGS) is a large-scale parallel sequencing that can sequence a large number of DNA molecules at the same time. It is a revolutionary progress after Sanger sequencing. It has high throughput, high sensitivity and specificity, can perform qualitative and quantitative detection, and the detection cost is relatively low. It has broad application prospects in non-invasive prenatal screening, tumor gene mutation and other fields, and is an important supporting technology in

The design of fluorescence quantitative PCR experiment needs to select relative quantification or absolute quantification method according to the purpose. Relative quantification is used to detect the relative expression difference of genes in different samples. The ΔΔCT method or relative standard curve method is commonly used. Appropriate internal reference genes need to be selected to eliminate human errors; absolute quantification is used to detect the accurate content of genes or species in

On the afternoon of November 30, Beijing Pukairui brought the Qihengxing qPCR Mix product to the Institute of Vegetables and Flowers of the Chinese Academy of Agricultural Sciences, and the conference was packed. The product manager introduced the principle of fluorescence quantitative PCR technology, the analytical method and the advantages of the Qihengxing qPCR Mix product, including higher fluorescence signal intensity, improved signal-to-noise ratio, strong amplification specificity, early

qPCR has become the gold standard for nucleic acid quantitative experiments due to its simple operation, rapid detection, high sensitivity and good specificity, and is widely used in life sciences, agronomy, molecular diagnosis and medicine. Nucleic acid extraction and quality control are the key initial links of qPCR experiments. Correct sample collection and processing methods, as well as effective nucleic acid extraction and strict quality control are important guarantees to ensure the accura

qPCR primer design follows the basic rules of PCR and varies depending on the quantitative method. When designing primers for the SYBR dye method, attention should be paid to the key points such as primer length, G+C content, 3' end base selection, avoidance of dimers and hairpin structures, Tm value matching, ΔG value control, 5' end modification, product length and intron spanning. In addition to the above principles, the TaqMan probe method also needs to pay attention to avoiding G bases at t