2025-01-16
As a revolutionary technology in the field of biological science, polymerase chain reaction (PCR) has been widely used in various fields such as gene cloning, mutation analysis, and disease diagnosis. The basic principle of PCR technology is an enzymatic synthesis reaction in an appropriate system in the presence of template DNA, DNA polymerase, four nucleotides, primers, necessary cofactors, etc. Inhibitors in the PCR reaction process will significantly reduce the sensitivity and amplification