StarRed Nucleic Acid Dye (10000 × )

StarRed is a highly sensitive, stable and safe fluorescent nucleic acid gel staining reagent. Its unique oily macromolecular structure prevents it from penetrating the cell membrane and entering the cell, and it is not easy to evaporate and sublimate and be inhaled by the human body.
StarRed has the same spectral characteristics as ethidium bromide (EtBr, EB). Its unique molecular structure can electrostatically bind to DNA without affecting the migration of DNA bands. Therefore, it can replace unsafe nucleic acid dyes such as EB.
It is suitable for staining dsDNA, ssDNA and RNA in agarose and polyacrylamide gel electrophoresis. You can choose gel staining or bubble staining for staining. It is very convenient and flexible to use.

• It is safe and non-toxic, has a large molecular weight, cannot penetrate cell membranes, and does not have the carcinogenicity and mutagenicity of EB.
• High sensitivity, suitable for electrophoretic staining of fragments of various sizes.
• High stability, dye can be added directly after agarose is heated and dissolved.
• Strong light resistance: stored in brown cryotubes, can be placed under normal light in the laboratory.
• The signal-to-noise ratio is good, the sample fluorescence signal is strong, and the background signal is low.

Note:

1. If the large molecular bands are tailing and the separation is not ideal, it is recommended to reduce the amount of DNA marker or nucleic acid sample loaded.
2. The gel staining method is not suitable for precast polyacrylamide gels. For polyacrylamide gels, please use the bubble staining method.
3. For your safety and health, please wear a lab coat and disposable gloves when operating.
4. This product is for scientific research purposes only!

TAE buffer preparation:
50X TAE electrophoresis buffer [Tris 242g (2M), EDTA 37.2g (100mM), add about 57ml of acetic acid to adjust pH=8.5; make up to 1000mL;
Use ddH ₂ Prepare 1X TAE electrophoresis buffer by diluting 50-fold.

TBE buffer configuration:
10X TBE electrophoresis buffer [Tris 107.8146 g (890 mM), boric acid 55.0287 g (890 mM), EDTA 5.845 g (20 mM), add about 4 g of NaOH to adjust pH = 8.3; constant volume 1000 mL];
Prepare 1X TBE electrophoresis buffer by diluting 10-fold with ddH2O.

1. Gel staining (same as EB, staining before electrophoresis)

1) Prepare agarose gel of appropriate concentration and heat it in a microwave oven until it is completely melted.

2) Add GelRed nucleic acid dye to a final concentration of 1× (i.e., add 5 μL of StarRed 10,000× aqueous solution to every 50 mL of agarose solution).

3) Pour the agarose solution containing Star Red nucleic acid dye into the gel casting apparatus and insert the comb. Let it solidify at room temperature for about 30-60 minutes.

4) Load the sample and perform electrophoresis according to conventional methods.

5) Ultraviolet photography and observation.

✮ Note : Star Red has good thermal stability and can be added directly to hot agarose solution, or Star Red stock solution can be added to electrophoresis buffer containing agarose powder.
Then heat in a microwave or other common way to prepare the agarose gel. Star Red is compatible with all common electrophoresis buffer solutions.

2. Foam staining (staining after electrophoresis)

1) Prepare agarose gel of appropriate concentration and heat it in a microwave oven until it is completely melted.

2) Pour the agarose solution into the gel casting apparatus and insert the comb. Let it solidify at room temperature for about 30-60 minutes.

3) Load the sample and perform electrophoresis according to conventional methods.

4) Dilute the StarRed 10,000× aqueous solution with 0.1 M NaCl solution to a 3× staining solution (i.e., add 15 μL of StarRed 10,000× aqueous solution to 50 mL of 0.1 M NaCl solution. The staining solution can be reused about 3 times and should be stored at room temperature away from light).

5) Place the gel in a suitable container and add 3× staining solution to submerge the gel. Stain at room temperature for about 30 minutes. The optimal staining time is related to the thickness and concentration of the gel. For gels containing 3.5-10% polyacrylamide,
Staining time usually ranges from 30 min to 1 h and increases with increasing polyacrylamide content.

6) UV photography and observation